State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
Ultrason Sonochem. 2020 Sep;66:105074. doi: 10.1016/j.ultsonch.2020.105074. Epub 2020 Mar 21.
With recent advances in nanotechnology, debranched starch nanoparticle (DBS-NP) materials have attracted considerable interest from the fields of functional food, biomedicine, and material science, thanks to their small size, biodegradability, biocompatibility, sustainability, and non-hazardous effects on health and the environment. In this study, DBS-NP was fabricated using an eco-friendly method involving ultrasonication combined with recrystallization. The effects of ultrasonication and recrystallization times on the morphology, particle size, and crystal structure of the DBS-NPs were systematically investigated. Compared with the DBS-NPs prepared using ultrasonication treatment only, the DBS-NPs formed using ultrasonication combined with recrystallization were uniform in size and well distributed in aqueous solution. Moreover, the maximum encapsulation efficiency and loading capacity of the epigallocatechin gallate (EGCG) in the DBS-NPs with ultrasonication treatment reached 88.35% and 22.75%, respectively. The particle sizes of the EGCG@DBS-NP were more stable at a neutral pH (7.4) than at an acidic pH (2.1). The EGCG in the EGCG@DBS-NP displayed excellent radical scavenging activity and antibacterial effects, and cell assays demonstrated that the EGCG@DBS-NP was non-toxic and highly biocompatible.
近年来，随着纳米技术的发展，支链淀粉纳米颗粒（DBS-NP）材料因其粒径小、可生物降解、生物相容性、可持续性以及对健康和环境无危害等特点，在功能食品、生物医药和材料科学等领域引起了广泛关注。本研究采用超声结合重结晶的环保方法制备了 DBS-NP，并系统研究了超声时间和重结晶时间对 DBS-NP 的形态、粒径和晶体结构的影响。与仅用超声处理制备的 DBS-NP 相比，超声结合重结晶制备的 DBS-NP 粒径均匀，在水溶液中分布良好。此外，用超声处理制备的 DBS-NP 对表没食子儿茶素没食子酸酯（EGCG）的最大包封效率和载药量分别达到 88.35%和 22.75%。在中性 pH（7.4）下，EGCG@DBS-NP 的粒径比在酸性 pH（2.1）下更稳定。EGCG@DBS-NP 中的 EGCG 具有优异的自由基清除活性和抗菌作用，细胞实验表明 EGCG@DBS-NP 无毒且具有高度的生物相容性。
