Chloroform mediated refractory state against ornithine decarboxylase induction by serial chloroform treatment.

The chloroform mediated refractory state against ornithine decarboxylase induction in male and female rat liver was further studied. One aspect of the investigation was to determine the duration of the induced refractory period while the other component focused on the extent to which the inhibitory effect was dependent upon the concentration of the first dose. When the dosing interval between the first and second dose was varied from 1 to 31 days, the magnitude of the resistance to further stimulation by chloroform only decreased gradually. In studies where the concentration of the first dose was varied while the dosing interval was fixed, it was concluded that the extent of the inhibitory effect was dependent upon the concentration of the first dose.