Chen Michael L, Becraft Eric D, Pachiadaki Maria, Brown Julia M, Jarett Jessica K, Gasol Josep M, Ravin Nikolai V, Moser Duane P, Nunoura Takuro, Herndl Gerhard J, Woyke Tanja, Stepanauskas Ramunas
Bigelow Laboratory for Ocean Sciences, East Boothbay, ME, United States.
Department of Biology, Williams College, Williamstown, MA, United States.
Front Microbiol. 2020 Mar 12;11:376. doi: 10.3389/fmicb.2020.00376. eCollection 2020.
Bacterial candidate phylum PAUC34f was originally discovered in marine sponges and is widely considered to be composed of sponge symbionts. Here, we report 21 single amplified genomes (SAGs) of PAUC34f from a variety of environments, including the dark ocean, lake sediments, and a terrestrial aquifer. The diverse origins of the SAGs and the results of metagenome fragment recruitment suggest that some PAUC34f lineages represent relatively abundant, free-living cells in environments other than sponge microbiomes, including the deep ocean. Both phylogenetic and biogeographic patterns, as well as genome content analyses suggest that PAUC34f associations with hosts evolved independently multiple times, while free-living lineages of PAUC34f are distinct and relatively abundant in a wide range of environments.
细菌候选门PAUC34f最初是在海洋海绵中发现的，被广泛认为由海绵共生体组成。在这里，我们报告了来自各种环境（包括黑暗海洋、湖泊沉积物和陆地含水层）的21个PAUC34f的单扩增基因组（SAG）。SAG的多样起源以及宏基因组片段招募结果表明，一些PAUC34f谱系代表了除海绵微生物群之外的其他环境中相对丰富的自由生活细胞，包括深海。系统发育和生物地理模式以及基因组内容分析都表明，PAUC34f与宿主的关联多次独立进化，而PAUC34f的自由生活谱系在广泛的环境中是独特且相对丰富的。
