Ko Suk-Min, Sun Hyeon-Jin, Oh Myung Jin, Song In-Ja, Kim Min-Jae, Sin Hyun-Sook, Goh Chang-Hyo, Kim Yong-Woo, Lim Pyung-Ok, Lee Hyo-Yeon, Kim Suk Weon
1Subtropical Horticulture Research Institute, Jeju National University, Jeju, 690-756 Korea.
2Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806 Korea.
Hortic Environ Biotechnol. 2011;52(5):511. doi: 10.1007/s13580-011-0007-x. Epub 2011 Oct 28.
Duckweeds are small, floating aquatic plants with a number of useful characteristics, including edibility, fast-growing, and a clonal proliferation. Duckweed is also fed to animals as a diet complement because of its high nutritional value. Porcine epidemic diarrhea virus (PEDV) is a major causative agent of fatal diarrhea in piglets and is a serious problem in the hog-raising industry. In this study, we assessed the feasibility of producing a protective antigen for the PEDV spike protein 1 using duckweed, . Stably transformed were obtained by co-cultivation with EHA105 harboring the PEDV spike protein gene. Transgene integration and expression of the PEDV spike protein 1 gene were confirmed by genomic PCR and RT-PCR and western blot analysis of transgenic , respectively. This is the first report of the expression of a vaccine antigen against an animal infectious disease in duckweed.
浮萍是小型漂浮水生植物，具有许多有用的特性，包括可食用、生长迅速和克隆增殖。由于其高营养价值，浮萍还被用作动物饲料的补充。猪流行性腹泻病毒（PEDV）是仔猪致命性腹泻的主要病原体，也是养猪业中的一个严重问题。在本研究中，我们评估了利用浮萍生产PEDV刺突蛋白1保护性抗原的可行性。通过与携带PEDV刺突蛋白基因的EHA105共培养获得了稳定转化体。分别通过基因组PCR、RT-PCR以及对转基因浮萍的western blot分析，证实了PEDV刺突蛋白1基因的转基因整合和表达。这是浮萍中表达针对动物传染病的疫苗抗原的首次报道。
