Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Sarawak State Health Department, Kuching, Malaysia.
Am J Trop Med Hyg. 2020 Jun;102(6):1370-1372. doi: 10.4269/ajtmh.20-0001.
The incidence of zoonotic malaria, , infection is increasing and now is the major cause of malaria in Malaysia. Here, we describe a WarmStart colorimetric loop-mediated isothermal amplification (LAMP) assay for the detection of spp. The detection limit for this assay was 10 copies/µL for and and 1 copy/µL for , , and . To test clinical sensitivity and specificity, 100 microscopy-positive and 20 malaria-negative samples were used. The WarmStart colorimetric LAMP was 98% sensitive and 100% specific. Amplification products were visible for direct observation, thereby eliminating the need for post-amplification processing steps. Therefore, WarmStart colorimetric LAMP is suitable for use in resource-limited settings.
动物源性疟疾的发病率不断上升，现已成为马来西亚疟疾的主要病因。在此，我们描述了一种 WarmStart 比色环介导等温扩增（LAMP）检测方法，用于检测 spp。该检测方法对 和 的检测限为 10 拷贝/μL，对 、 、 和 的检测限为 1 拷贝/μL。为了测试临床灵敏度和特异性，我们使用了 100 份显微镜阳性和 20 份疟疾阴性样本。WarmStart 比色 LAMP 的灵敏度为 98%，特异性为 100%。扩增产物可直接观察到，因此无需进行扩增后处理步骤。因此，WarmStart 比色 LAMP 适用于资源有限的环境。
