Departamento de Microbiologia - Instituto Aggeu Magalhães, Fiocruz, Recife, Pernambuco, Brazil; Centro Universitário Vale do Ipojuca - Unifavip/Wyden, Caruaru, Pernambuco, Brazil.
Departamento de Microbiologia - Instituto Aggeu Magalhães, Fiocruz, Recife, Pernambuco, Brazil.
J Microbiol Methods. 2020 May;172:105903. doi: 10.1016/j.mimet.2020.105903. Epub 2020 Mar 27.
The Yersinia pestis capsular antigen F1 is widely used in plague laboratory diagnosis. Here, we describe the production of an F1 recombinant protein within reduced time and biosafety requirements. Its evaluation in hemagglutination tests indicated that the recombinant F1 can replace the conventional F1 protein for plague diagnosis.
鼠疫耶尔森氏菌荚膜抗原 F1 被广泛用于鼠疫的实验室诊断。在此,我们描述了一种在满足减少时间和生物安全要求的情况下生产 F1 重组蛋白的方法。其在血凝试验中的评估表明,重组 F1 可以替代常规的 F1 蛋白用于鼠疫诊断。