Department of Physical and Environmental Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, Canada.
Biosensors (Basel). 2020 Mar 27;10(4):30. doi: 10.3390/bios10040030.
Herein, graphene oxide nanoribbons (GONRs) were obtained from the oxidative unzipping of multi-walled carbon nanotubes. Covalent coupling reaction of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxy succinimide (NHS) with amine functional groups (-NH) of the chitosan natural polymer (CH) was used for entrapping GONRs on the activated glassy carbon electrode (GCE/GONRs-CH). The nanocomposite was characterized by high-resolution transmission electron microscopy (HRTEM), and field-emission scanning electron microscopy (FESEM). In addition, the modification steps were monitored using FTIR. The nanocomposite-modified electrode was used for the simultaneous electrochemical determination of four DNA bases; guanine (G), adenine (A), thymine (T) and cytosine (C). The nanocomposite-modified GCE displayed a strong, stable and continuous four oxidation peaks during electrochemistry detection at potentials 0.63, 0.89, 1.13 and 1.27 V for G, A, T and C, respectively. The calibration curves were linear up to 256, 172, 855 and 342 μM with detection limits of 0.002, 0.023, 1.330 and 0.641 μM for G, A, T and C, respectively. The analytical performance of the GCE/GONRs-CH has been used for the determination of G, A, T and C in real samples and obtained a recovery percentage from 91.1%-104.7%. Our preliminary results demonstrated that GCE/GONRs-CH provided a promising platform to detect all four DNA bases for future studies on DNA damage and mutations.
在此，通过多壁碳纳米管的氧化解旋得到了氧化石墨烯纳米带（GONRs）。1-乙基-3-（3-二甲基氨基丙基）碳二亚胺盐酸盐（EDC）和N-羟基琥珀酰亚胺（NHS）与壳聚糖天然聚合物（CH）中的氨基官能团（-NH）的共价偶联反应用于将 GONRs 包埋在活化的玻碳电极（GCE/GONRs-CH）上。该纳米复合材料通过高分辨率透射电子显微镜（HRTEM）和场发射扫描电子显微镜（FESEM）进行了表征。此外，使用傅里叶变换红外光谱（FTIR）监测了修饰步骤。将纳米复合材料修饰的电极用于同时电化学测定四种 DNA 碱基；鸟嘌呤（G）、腺嘌呤（A）、胸腺嘧啶（T）和胞嘧啶（C）。在电化学检测中，纳米复合材料修饰的 GCE 在 0.63、0.89、1.13 和 1.27 V 时分别显示出 G、A、T 和 C 的四个强、稳定和连续的氧化峰。校准曲线在 256、172、855 和 342 μM 范围内呈线性，G、A、T 和 C 的检测限分别为 0.002、0.023、1.330 和 0.641 μM。GCE/GONRs-CH 的分析性能已用于实际样品中 G、A、T 和 C 的测定，回收率为 91.1%-104.7%。我们的初步结果表明，GCE/GONRs-CH 为未来的 DNA 损伤和突变研究提供了一个有前途的检测所有四种 DNA 碱基的平台。
