从印度野生型斑马鱼（Danio rerio （Hamilton 1822））中开发和鉴定一种新的 DRCF 细胞系。
Development and characterization of a new DRCF cell line from Indian wild strain zebrafish Danio rerio (Hamilton 1822).
机构信息
Fish Genetics & Biotechnology Division, ICAR - Central Institute of Fisheries Education, Panch Marg, Off. Yari Road, Mumbai, 400061, India.
Department of Biotechnology, Netaji Subhash Institute of Technology, Azad Hind Fauz Marg, Dwarka Sector-3, New Delhi, India.
出版信息
Fish Physiol Biochem. 2020 Aug;46(4):1337-1347. doi: 10.1007/s10695-020-00792-x. Epub 2020 Mar 30.
Danio rerio, zebrafish, has been widely used as a non-mammalian vertebrate model organism in various studies. The present research describes to develop and characterize a new cell line from a wild strain Indian zebrafish native to Brahmaputra River, Assam, India. The new cell line designated as DRCF was developed from the caudal fin of D. rerio. The cell line was successfully subcultured up to 31 passages. Growth studies revealed that cell growth of DRCF was optimal at 28 °C in L-15 medium supplemented with 20% FBS. Molecular characterization of the DRCF cell line using mitochondrial genes namely cytochrome oxidase subunit I gene (COI) and 16S rRNA authenticated the true origin of the cell line. The chromosome analysis of the DRCF cell line expressed its 50 diploid chromosome number of D. rerio. The immunocytochemical characterization of the cell line exhibited its fibroblastic morphology. The expression of the green fluorescent protein (GFP) following transfection revealed the suitability of the cell line for transfection studies.
斑马鱼（Danio rerio）是一种广泛应用于各种研究的非哺乳动物脊椎动物模式生物。本研究描述了从印度 Assam 的 Brahmaputra 河的野生印度斑马鱼中开发和鉴定一种新的细胞系。新的细胞系命名为 DRCF，源自斑马鱼的尾鳍。该细胞系成功地传代培养至 31 代。生长研究表明，DRCF 细胞在添加 20% FBS 的 L-15 培养基中，在 28°C 时生长最佳。使用线粒体基因（即细胞色素氧化酶亚基 I 基因（COI）和 16S rRNA）对 DRCF 细胞系进行的分子特征分析证实了该细胞系的真实来源。DRCF 细胞系的染色体分析表达了其 50 条二倍体染色体数目的斑马鱼。细胞系的免疫细胞化学特征显示其成纤维细胞形态。转染后的绿色荧光蛋白（GFP）表达表明该细胞系适合转染研究。
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