Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan.
Department of Basic Veterinary Medical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan.
Pol J Vet Sci. 2020 Mar;23(1):133-141. doi: 10.24425/pjvs.2020.132757.
The objectives of this study were to determine the genetic relatedness, antibiogram and virulence factors of Staphylococcus aureus (S. aureus) isolated from bovine mastitis, associated farm workers, dairy cow farm veterinarians (private veterinarians), veterinary students, and non-veterinary university students. A total of 84 S. aureus isolates (27 from mastitis, 11 from farm workers, 9 from private veterinarians, 22 from veterinary students, and 16 from non-veterinary university students) were used to determine antimicrobial sensitivity patterns using disk diffusion test, virulence factors using PCR technique and phylogenic analysis using pulsed field gel electrophoresis. All S. aureus isolates were resistant to 2 or more commonly used antibiotics. All isolates from mastitis, farm workers, and veterinary students carried the genes encoding coagulase and thermonuclease factors while isolates from non-veterinary university students carried the genes encoding coagulase, clumping, and thermonuclease factors. The mecA gene was detected in 22.2%, 81.8%, 100%, 95.5% and 100% of isolates from mastitis, farm workers, private veterinarians, veterinary students, and non-veterinary university students, respectively. In the phylogenic analysis, 10 (45.5%), 6 (66.7%) and 8 (72.7%) isolates from veterinary students, private veterinarians and farm workers, respectively were more than 80% similar to isolates from mastitis. Results of this study indicate that S. aureus isolates from mastitis milk and those from related dairy cow personnel and veterinarians share similar antimicrobial sensitivity patterns and virulence factors, therefore a common source of bacteria may exist. Furthermore, possible transmission of S. aureus between cows and cow-related personnel and vice versa could also exist.
本研究的目的是确定从奶牛乳腺炎、相关农场工人、奶牛场兽医（私人兽医）、兽医学生和非兽医大学生中分离出的金黄色葡萄球菌（S. aureus）的遗传关系、抗生素谱和毒力因子。共使用 84 株 S. aureus 分离株（27 株来自乳腺炎，11 株来自农场工人，9 株来自私人兽医，22 株来自兽医学生，16 株来自非兽医大学生），采用纸片扩散法测定抗菌敏感性模式，采用 PCR 技术测定毒力因子，采用脉冲场凝胶电泳进行系统发育分析。所有 S. aureus 分离株均对 2 种或 2 种以上常用抗生素耐药。乳腺炎、农场工人和兽医学生的所有分离株均携带编码凝固酶和热核酸酶因子的基因，而非兽医大学生的分离株携带编码凝固酶、聚集和热核酸酶因子的基因。mecA 基因在乳腺炎、农场工人和兽医学生的分离株中的检出率分别为 22.2%、81.8%、100%、95.5%和 100%，在私人兽医和兽医学生的分离株中的检出率分别为 81.8%、100%、95.5%和 100%，在非兽医大学生的分离株中的检出率为 100%。在系统发育分析中，兽医学生、私人兽医和农场工人的 10（45.5%）、6（66.7%）和 8（72.7%）株分离株与乳腺炎分离株的相似度均超过 80%。本研究结果表明，乳腺炎奶中分离出的金黄色葡萄球菌株及其相关奶牛人员和兽医具有相似的抗菌敏感性模式和毒力因子，因此可能存在细菌的共同来源。此外，金黄色葡萄球菌可能在奶牛与奶牛相关人员之间以及反之亦然进行传播。
