Giavazzi R, Garofalo A
Mario Negri Institute for Pharmacological Research, Bergamo, Italy.
Adv Exp Med Biol. 1988;233:39-47. doi: 10.1007/978-1-4899-5037-6_6.
The tumorigenicity and metastatic behaviour of human colorectal tumors have been assessed in athymic nude mice. Surgical specimens were obtained from colorectal carcinomas or from metastases of different patients. Primary colorectal tumors and their synchronous liver metastases were obtained from three patients. After intramuscular (i.m.) injection into nude mice, tumor cells originating from metastases showed a higher take than those from primary tumors. In general, metastasis-derived tumors had a shorter doubling time than primary-derived tumors. This growth pattern was not associated with the production of spontaneous metastases in nude mice. To investigate the malignant potential of colorectal neoplasms in the nude mouse, tumor cells were injected intravenously (i.v.) (artificial lung metastases) and in the spleen (artificial liver metastases). Colorectal-derived tumor cells produced lung colonies after i.v. injection, but the most dramatic expression of malignancy was the tumor cells' ability to grow in the liver after intrasplenic (i.s.) injection. The ability to grow in the liver of nude mice appeared to be a distinct characteristic of each tumor cell population. Human colorectal tumors were maintained as continuous lines subcutaneously (s.c.) in nude mice. Phenotypic differences, including malignant potential, were generally maintained at different passages. Human colorectal tumors with a definite pattern of malignant behaviour (i.e. to the liver) in the nude mouse provide a unique experimental system for the study of the biology and the therapy of cancer metastases.
已在无胸腺裸鼠中评估了人类结直肠肿瘤的致瘤性和转移行为。手术标本取自不同患者的结直肠癌或转移灶。从三名患者身上获取了原发性结直肠肿瘤及其同步性肝转移灶。将肿瘤细胞肌内注射到裸鼠体内后，源自转移灶的肿瘤细胞比源自原发性肿瘤的肿瘤细胞具有更高的接种成功率。一般来说，转移灶来源的肿瘤比原发性肿瘤来源的肿瘤倍增时间更短。这种生长模式与裸鼠中自发转移的产生无关。为了研究结直肠肿瘤在裸鼠中的恶性潜能，将肿瘤细胞静脉注射（人工肺转移）和注射到脾脏中（人工肝转移）。结直肠来源的肿瘤细胞静脉注射后产生肺集落，但恶性程度最显著的表现是肿瘤细胞脾内注射后在肝脏中生长的能力。在裸鼠肝脏中生长的能力似乎是每个肿瘤细胞群体的一个独特特征。人类结直肠肿瘤在裸鼠皮下维持为连续传代细胞系。包括恶性潜能在内的表型差异在不同传代中通常得以维持。在裸鼠中具有明确恶性行为模式（即转移至肝脏）的人类结直肠肿瘤为癌症转移生物学和治疗研究提供了一个独特的实验系统。
