采用 LC-MS/MS 法测定猕猴桃中吡虫啉的消解动态和残留。
Determination of nitenpyram dissipation and residue in kiwifruit by LC-MS/MS.
机构信息
State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University , Guiyang, P. R. China.
出版信息
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2020 Jun;37(6):955-962. doi: 10.1080/19440049.2020.1741693. Epub 2020 Apr 1.
To investigate the presence of nitenpyram in kiwifruit, a field experiment was conducted in six kiwifruit producing regions in China. A modified quick, easy, cheap, effective, rugged and safe method (QuEChERS) method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the determination of nitenpyram in kiwifruit. Quantitative detection was performed by LC-MS/MS in multiple reaction monitoring (MRM) mode under positive-ion electrospray ionisation. The proposed method is linear and accurate in the range of 0.005 to 4.5 μg/mL. The recovery of nitenpyram was satisfactory (71.96-96.67%) with reasonable RSDs (9.83 %) for 0.01-4.5 mg/kg spiked levels. The limit of quantification (LOQ) was 0.01 mg/kg for nitenpyram. Nitenpyram degraded rapidly in kiwifruit with a half-life <4 days. Residues of nitenpyram were <0.01 - 0.54 mg/kg in kiwifruit 7, 14 and 21 days after spraying at six sites.
为了研究噻虫嗪在猕猴桃中的存在情况，在中国 6 个猕猴桃产区进行了田间试验。建立了一种基于液相色谱-串联质谱（LC-MS/MS）的改良的快速、简便、廉价、有效、耐用和安全方法（QuEChERS），用于测定猕猴桃中的噻虫嗪。采用正离子电喷雾电离多反应监测（MRM）模式，通过 LC-MS/MS 进行定量检测。该方法在 0.005 至 4.5 μg/mL 范围内呈线性且准确。噻虫嗪在 0.01-4.5 mg/kg 加标水平下的回收率令人满意（71.96-96.67%），相对标准偏差（RSD）合理（9.83%）。噻虫嗪的定量限（LOQ）为 0.01 mg/kg。噻虫嗪在猕猴桃中降解迅速，半衰期<4 天。在 6 个地点施药 7、14 和 21 天后，猕猴桃中噻虫嗪的残留量<0.01-0.54 mg/kg。
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