Institute of Biology Systems and Genetic Research, Veterinary Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania
Department of Otorhinolaryngology, Lithuanian University of Health Sciences, Kaunas, Lithuania.
Anticancer Res. 2020 Apr;40(4):2003-2009. doi: 10.21873/anticanres.14156.
BACKGROUND/AIM: Matrix metalloproteinases (MMPs) are a family of proteins which are involved in breakdown of the extracellular matrix in embryonic development, tissue remodeling and in some diseases. MMP8 has both cancer-promoting and anticancer properties. However, the contribution of MMP8 to laryngeal squamous cell carcinoma (LSCC) has not been elucidated. In this study we aimed to test the contribution of two MMP8 polymorphisms, located in the gene promoter region, to the development of LSCC.
This case-control study involved 569 DNA samples which were genotyped for two single nucleotide polymorphisms using real-time polymerase chain reaction method. Statistical analysis was performed with SPSS Statistics 20 software.
Regression analysis adjusted by age showed that for MMP8 rs11225395 each minor A allele copy significantly reduced the odds for LSCC development (odds ratio=0.49, 95% confidence intervaI=0.04-2.19, p=0.048). MMP8 rs11225395 AA genotype was associated with smaller laryngeal tumour size (p=0.023). Smoking habit also correlated with laryngeal tumor size.
MMP8 rs11225395 and smoking habits have a prominent interface with LSCC tumour size.
背景/目的：基质金属蛋白酶（MMPs）是一类参与胚胎发育、组织重塑和某些疾病中细胞外基质分解的蛋白质。MMP8 具有促进癌症和抗癌的特性。然而，MMP8 对喉鳞状细胞癌（LSCC）的贡献尚未阐明。本研究旨在检测基因启动子区域两个 MMP8 多态性与 LSCC 发展的关系。
本病例对照研究共涉及 569 份 DNA 样本，采用实时聚合酶链反应方法对两种单核苷酸多态性进行基因分型。采用 SPSS Statistics 20 软件进行统计分析。
调整年龄的回归分析表明，对于 MMP8 rs11225395，每个次要 A 等位基因拷贝显著降低了 LSCC 发展的几率（比值比=0.49，95%置信区间=0.04-2.19，p=0.048）。MMP8 rs11225395 AA 基因型与较小的喉肿瘤大小相关（p=0.023）。吸烟习惯也与喉肿瘤大小相关。
MMP8 rs11225395 和吸烟习惯与 LSCC 肿瘤大小有显著的相关性。
