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一个基因的表达延迟了开花时间并增强了对压力的耐受性。

Expression of a Gene from Delays Flowering Time and Enhances Stress Tolerance.

机构信息

College of Grassland Science, Beijing Forestry University, Beijing 100083, China.

College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing 210095, China.

出版信息

Int J Mol Sci. 2020 Mar 30;21(7):2384. doi: 10.3390/ijms21072384.

Abstract

Shoot branching is one of the most variable determinants of crop yield, and the signaling pathways of plant branches have become a hot research topic. As an important transcription factor in the B3 family, NGATHA1 (NGA1), plays an important role in regulating plant lateral organ development and hormone synthesis and transport, but few studies of the role of this gene in the regulation of plant growth and stress tolerance have been reported. In this study, the gene was isolated from and its function was characterized. The cis-acting elements upstream of the 5' end of and the expression pattern of were analyzed, and the results indicated that the gene may act as a regulator of stress resistance. A plant expression vector was constructed and transgenic Arabidopsis plants were obtained. Transgenic Arabidopsis showed delayed flowering time and reduced branching phenotypes. Genes involved in the regulation of branching and flowering were differentially expressed in transgenic plants compared with wild-type plants. Furthermore, transgenic plants demonstrated strong tolerances to salt- and mannitol-induced stresses, which may be due to the upregulated expression of () by the gene. These results provide useful information for the exploration and genetic modification use of in the future.

摘要

分枝是作物产量最具变异性的决定因素之一,植物分枝的信号通路已成为一个热门研究课题。作为 B3 家族中的一个重要转录因子,NGATHA1(NGA1)在调节植物侧生器官发育和激素合成与运输方面发挥着重要作用,但关于该基因在调节植物生长和抗逆性方面的作用的研究较少。本研究从 中分离出该基因,并对其功能进行了鉴定。分析了 5'端上游顺式作用元件和 的表达模式,结果表明该基因可能作为抗逆性的调节因子发挥作用。构建了植物表达载体,并获得了转基因拟南芥植株。与野生型植株相比,转基因拟南芥开花时间延迟,分枝表型减少。与分枝和开花调控相关的基因在转基因植株中呈现差异表达。此外,转基因植株对盐和甘露醇诱导的胁迫具有较强的耐受性,这可能是由于 基因上调了 ()的表达。这些结果为未来探索和遗传修饰 提供了有用的信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd7c/7177866/a91a5d3b22c8/ijms-21-02384-g001.jpg

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