College of Chemistry and Chemical Engineering, Xinjiang University, Urumqi, 830046, China.
J Food Sci. 2020 Apr;85(4):947-955. doi: 10.1111/1750-3841.15093. Epub 2020 Apr 1.
Edible packaging films have been widely studied because of its safety, green, and effective characteristics. In this paper, chitosan (CH) edible films containing hexahydro-β-acids (HBA) were prepared, and its physical and mechanical properties, bioactivity, and their impact on the shelf life of pork were investigated. The infrared spectra indicated that the molecular interaction between CH and HBA was observed. Scanning electron microscopy was used to analyze the surface morphology of the film, and light transmittance analysis displayed that the addition of HBA enhanced the film's UV blocking performance. Compared to the CH film, the tensile strength of CH-HBA film increased to 29.19 ± 0.45 MPa, and the scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) reached 1.40 ± 0.01 mg rutin/cm of the film. The antibacterial activity of the CH-HBA film on Escherichia coli (44825) and Staphylococcus aureus (26001) showed that the CH-HBA film is a feasible antibacterial package. Furthermore, compared to pork packaged in CH and polyethylene films, fresh pork packaged with CH-HBA films displayed prolongation of shelf life due to reduction in microbial proliferation, thiobarbituric values, pH, and total volatile base nitrogen contents during storage at 4 °C for 16 days. The freshness of pork was prolonged by 7-8 days when the dosage of HBA was increased to 0.3% from 0.1% (w/v). These results revealed that the CH-HBA film can effectively extend the shelf life of pork. PRACTICAL APPLICATION: This study effectively prolonged the shelf life of pork. A chitosan-edible film combined with hexahydro-β-acids has a potential application value in replacing traditional packaged fresh meat.
可食用包装薄膜因其安全、绿色和有效等特点而受到广泛研究。本文制备了含有六氢-β-酸（HBA）的壳聚糖（CH）可食用膜，并研究了其物理力学性能、生物活性及其对猪肉货架期的影响。红外光谱表明观察到 CH 和 HBA 之间的分子相互作用。扫描电子显微镜用于分析膜的表面形态，透光率分析显示添加 HBA 增强了膜的紫外阻断性能。与 CH 膜相比，CH-HBA 膜的拉伸强度增加到 29.19±0.45 MPa，2,2-二苯基-1-苦基肼（DPPH）的清除活性达到 1.40±0.01 mg 芦丁/cm 膜。CH-HBA 膜对大肠杆菌（44825）和金黄色葡萄球菌（26001）的抗菌活性表明，CH-HBA 膜是一种可行的抗菌包装。此外，与在 CH 和聚乙烯薄膜包装的新鲜猪肉相比，在 4°C 下储存 16 天时，用 CH-HBA 薄膜包装的新鲜猪肉由于微生物增殖、硫代巴比妥酸值、pH 值和总挥发性碱基氮含量的减少，货架寿命延长。当 HBA 的用量从 0.1%（w/v）增加到 0.3%时，猪肉的新鲜度延长了 7-8 天。这些结果表明，CH-HBA 膜可以有效延长猪肉的货架期。实际应用：本研究有效延长了猪肉的货架期。壳聚糖-可食用膜与六氢-β-酸的结合具有替代传统包装鲜肉的潜在应用价值。
