Hua Hua, Yan Zhi-Xiang, Tian Wei-Wei, Liu Li, Sun Hong-Bing, Zhou Xian-Jian, Yan Liang-Chun, Zhao Jun-Ning
Institute of Translational Pharmacology and Clinical Application, Sichuan Center for Translational Medicine of Traditional Chinese Medicine, Sichuan Engineering Technology Research Center of Genuine Regional Drug, Biological Assay Key Laboratory of State Administration of Traditional Chinese Medicine for Traditional Chinese Medicine Quality, Sichuan Provincial Key Laboratory of Quality Evaluation and New Drug Creation of Traditional Chinese Medicine, Laboratory for Rapid Detection of Toxicity of Traditional Chinese Medicine in Sichuan Provincial Administration of Chinese Medicine, Sichuan Academy of Chinese Medicine Sciences Chengdu 610041, China.
Zhongguo Zhong Yao Za Zhi. 2020 Feb;45(4):732-738. doi: 10.19540/j.cnki.cjcmm.20191226.102.
"Huajiao" is dried ripe fruit peel of Zanthoxylum bungeanum or Z. schinifolium, is konwn as geoherbs, especially the "Dahongpao" cultivated in Hanyuan, Maoxian and Jiulong of Sichuan province. However, the genetic basis of Dao-di "Huajiao" is virtually unknown. The transcriptome of the fruit and leaf from Sichuan(Hanyuan, Jiulong, Lixian, Maoxian), Gansu(Wudu) province and Shaanxi(Fengxian) province was sequenced. Trinity de novo assembling resulted in a total of 177 616 unigenes. Through the KEGG, NR, SwissProt, Trembl, KOG/COG, GO, Pfam database comparision 106 644 annotated Unigene finally, 4 574 deferentially expressed genes were found in fruit between Sichuan and other provinces, including 3 740 up-regulated genes and 834 down-regulated genes. Among the up-regulated genes, 27 up-regulated genes were raleted to terpenoids, and 8 up-regulated genes were related to isoquinoline alkaloid bio-synthesis. Furthermore, it was also showed remarkable differences in groups which enrichment ratio of the diffe-rent expressed gene compared. The different expressed genes were annotated by the KEGG database into plant-pathogen interaction, plant hormone signal transduction and phenylpropanoid biosynthesis in fruit and leaf, but isoflavonoid bio-synthesis and betaine bio-synthesis were significantly different in fruit and leaf. The study laid a certain reference basis for comparison of quality and different expressed gene of Z. bungeanum from different groups.
“花椒”是芸香科植物花椒或竹叶花椒的干燥成熟果皮，为道地药材，尤以四川省汉源、茂县、九龙所产“大红袍”而闻名。然而，道地“花椒”的遗传基础却几乎无人知晓。对来自四川（汉源、九龙、理县、茂县）、甘肃（武都）和陕西（凤县）的果实和叶片进行转录组测序。Trinity从头组装共得到177616个单基因。通过KEGG、NR、SwissProt、Trembl、KOG/COG、GO、Pfam数据库比对，最终注释得到106644个单基因，发现四川与其他省份果实间有4574个差异表达基因，其中上调基因3740个，下调基因834个。上调基因中，27个上调基因与萜类化合物相关，8个上调基因与异喹啉生物碱生物合成相关。此外，不同表达基因富集率比较的组间也表现出显著差异。差异表达基因通过KEGG数据库注释到果实和叶片中的植物-病原体相互作用、植物激素信号转导和苯丙烷生物合成途径，但果实和叶片中的异黄酮生物合成和甜菜碱生物合成存在显著差异。该研究为不同群体花椒品质及差异表达基因比较提供了一定的参考依据。
