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CO γ 射线诱导豇豆荚性状的特征——有希望的产量突变体。

Characterization of CO γ-ray induced pod trait of blackgram-A promising yield mutants.

机构信息

Division of Crop Mutation and Molecular Breeding, Department of Botany, School of Life Sciences, Periyar University, Salem, India.

出版信息

Int J Radiat Biol. 2020 Jul;96(7):929-936. doi: 10.1080/09553002.2020.1748738. Epub 2020 Apr 13.

Abstract

Blackgram ( (L.) Hepper) possesses natural flower dropping character with self-pollination, which was highly challenging to create variability through hybridization. Gamma irradiation is a powerful tool to induce genetic alteration and improvement in crops with beneficial mutants. Hence, the present investigation focused on finding out the mutant characters related to the improvement of the agronomic characters and yield-related traits by using gamma irradiation. Pod mutant isolated with fruitful agronomic trait at M (Mutagenic) generation was identified and isolated by gamma irradiation is a central focus in the present study, which enhances the yield of the blackgram. Viable and uniform size of blackgram Vamban-4 seeds were irradiated at a different dose of CO (Cobalt) γ-rays 200, 400, 600, 800, 1000, and 1200 Gray (Gy). The further generations were screened and raised to M and M plants to select the stable mutants with yield traits. In the M generation, the following viable and pod mutants were identified, such as tall, dwarf, multipod, small pod, bold pod, striata pod. Morphological traits, such as plant height (PH), number of branches per plant (NBP), number of leaves per plant (NLP), number of cluster per plant (NCP), number of pods per plant (NPP), number of seeds per pod (NSP), pod length (PL), a hundred seed weight (HSW), seed yield per plant (SYP), were measured. Among the mutants, the increased morphogenetic traits were recorded in tall mutants (600 Gy), and the enhancement of yield characteristics noticed in multipod (800 Gy) and bold pod mutants (600 Gy). Compared to other doses, 600 Gy shows enhanced quantitative traits for blackgram improvement. The CO γ-rays induced genetic variation in the yield and its related traits and obtained pod mutants, which are useful to enhance the commercial value of species and crop improvement.

摘要

兵豆((L.)Hepper)具有天然的落花自交特性,通过杂交很难产生变异性。伽马射线辐照是一种强大的工具,可以诱导具有有益突变体的作物的遗传改变和改良。因此,本研究的重点是通过伽马射线辐照发现与提高农艺性状和产量相关性状相关的突变体特征。在 M(诱变)代中,分离出具有有益农艺性状的荚果突变体,并通过伽马射线辐照进行鉴定和分离,这是本研究的一个重点,它提高了兵豆的产量。将大小均匀、活力强的兵豆 Vamban-4 种子用不同剂量的钴(Co)γ射线 200、400、600、800、1000 和 1200 戈瑞(Gy)进行辐照。然后对后代进行筛选和培养,以 M 和 M 代植株的形式选择具有产量性状的稳定突变体。在 M 代中,鉴定出以下有活力的和荚果突变体,如高、矮、多荚、小荚、粗荚、条纹荚。对形态特征,如株高(PH)、每株分枝数(NBP)、每株叶数(NLP)、每株簇数(NCP)、每株荚数(NPP)、每荚种子数(NSP)、荚长(PL)、百粒重(HSW)、单株种子产量(SYP)进行测量。在这些突变体中,高突变体(600 Gy)的形态发生特性增加,多荚突变体(800 Gy)和粗荚突变体(600 Gy)的产量特征增强。与其他剂量相比,600 Gy 对提高兵豆的数量性状有更好的效果。CO 伽马射线辐照在产量及其相关性状中诱导了遗传变异,并获得了荚果突变体,这有助于提高物种和作物改良的商业价值。

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