两步法细菌人工染色体 (BAC) 工程：用重组穿梭载体电转化感受态 BAC 宿主细胞。
Two-Step Bacterial Artificial Chromosome (BAC) Engineering: Electroporation of Competent BAC Host Cells with the Recombinant Shuttle Vector.
出版信息
Cold Spring Harb Protoc. 2020 Apr 1;2020(4):098079. doi: 10.1101/pdb.prot098079.
PMID:32238593
Abstract
Bacterial artificial chromosome (BAC) clones are rendered electrocompetent and transformed with the recombinant shuttle vector, pLD53SCAB/AB-box. Cointegrates are selected by growth on chloramphenicol and ampicillin to ensure recombination of the shuttle vector into the BAC.
摘要
细菌人工染色体 (BAC) 克隆转化为感受态细胞，并与重组穿梭载体 pLD53SCAB/AB-box 共转化。通过在氯霉素和氨苄青霉素上的生长来选择共整合体，以确保穿梭载体重组到 BAC 中。
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