Cold Spring Harb Protoc. 2020 Apr 1;2020(4):099325. doi: 10.1101/pdb.prot099325.
There are many uses for antibodies labeled with metal ions. Most of these methods involve first attaching a metal chelator to the antibody molecule. This is achieved using standard cross-linking chemistry and then adding the desired metal at appropriate concentration and pH. The method described here outlines a basic procedure for creating a lanthanide conjugate. Lanthanide conjugates are used for proximity assays, as MRI contrast agents, or for mass cytometry experiments. Different metals and chelators can be substituted, but the basic procedures are similar.
抗体标记金属离子有很多用途。这些方法大多涉及首先将金属螯合剂连接到抗体分子上。这是通过使用标准的交联化学方法来实现的，然后在适当的浓度和 pH 值下添加所需的金属。这里描述的方法概述了创建镧系元素缀合物的基本程序。镧系元素缀合物用于邻近测定、磁共振成像对比剂或质谱细胞术实验。可以替代不同的金属和螯合剂，但基本程序是相似的。
