人血清生物素酶作为生物素结合蛋白的作用。
Role of human serum biotinidase as biotin-binding protein.
作者信息
Chauhan J, Dakshinamurti K
机构信息
Department of Biochemistry, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
出版信息
Biochem J. 1988 Nov 15;256(1):265-70. doi: 10.1042/bj2560265.
Abstract
Biotinidase shows two binding sites for biotin, with Kd = 59 and 3 nM respectively, and requires tryptophan and cysteine residues of the biotinidase protein for biotin-binding activity. Analysis of human serum by various column-chromatographic techniques indicates that biotinidase is the only protein which exchanges with labelled (+)-biotin. It was shown previously that epileptic patients receiving a high average dose of anticonvulsants (containing a carbamide group) have lower biotin concentrations than those receiving a low dose. We have shown in human serum and with purified biotinidase that these anticonvulsant drugs compete with biotin for binding to the protein moiety.
摘要
生物素酶显示出两个生物素结合位点，其解离常数（Kd）分别为59 nM和3 nM，并且生物素结合活性需要生物素酶蛋白中的色氨酸和半胱氨酸残基。采用各种柱色谱技术分析人血清表明，生物素酶是唯一能与标记的（+）-生物素进行交换的蛋白质。先前有研究表明，接受高平均剂量抗惊厥药（含尿素基团）的癫痫患者，其生物素浓度低于接受低剂量抗惊厥药的患者。我们在人血清和纯化的生物素酶中均已表明，这些抗惊厥药物与生物素竞争结合蛋白部分。
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