Møller J C
Department of Cell Biology, University of Aarhus, Denmark.
APMIS Suppl. 1988;4:82-6.
The aim of this study was to determine the effects of long-term compensatory growth (CRG) on the dimensions and ultrastructure of the renal proximal tubules. CRG was induced in right kidneys of pigs by subjecting the contralateral (left) kidneys total ureteral obstruction for 10 weeks. The length density (Lv) of proximal tubules was assessed at the light-microscopical level. Peritubular and luminal diameters, volume density (Vv) of mitochondria and surface density (Sv) of basolateral cell membranes were determined on montages of electron micrographs representing entire tubular cross-sections. Kidneys with CRG showed an average increase in weight of approximately 36%. The proximal tubules had near normal cross-sectional dimensions, surface density of basolateral cell membranes and volume density of mitochondria. The length density of proximal tubules was also the same as in control kidneys. However, there was an estimated increase in total proximal tubular length of almost 45%. The present results, combined with observations from short-term studies of CRG, suggest that the proximal tubules undergo a series of structural changes initiated by hypertrophy of cells and organelles and resulting in a state of predominant hyperplasia.
本研究的目的是确定长期代偿性生长（CRG）对肾近端小管尺寸和超微结构的影响。通过对猪的对侧（左）肾进行全输尿管梗阻10周，诱导右侧肾发生CRG。在光学显微镜水平评估近端小管的长度密度（Lv）。在代表整个肾小管横截面的电子显微镜照片蒙片上，测定肾小管周围和管腔直径、线粒体的体积密度（Vv）以及基底外侧细胞膜的表面密度（Sv）。发生CRG的肾脏平均重量增加约36%。近端小管的横截面尺寸、基底外侧细胞膜的表面密度和线粒体的体积密度接近正常。近端小管的长度密度也与对照肾脏相同。然而，据估计近端小管的总长度增加了近45%。目前的结果与CRG短期研究的观察结果相结合，表明近端小管经历了一系列由细胞和细胞器肥大引发并导致主要为增生状态的结构变化。
