Kone B C, Madsen K M, Tisher C C
Am J Anat. 1984 Oct;171(2):217-26. doi: 10.1002/aja.1001710207.
The thick ascending limb of Henle (TAL) in the rat until recently has been considered a morphologically homogeneous structure despite physiologic and biochemical evidence to the contrary. The present study was designed to examine the ultrastructural characteristics of the TAL in the inner cortex and the outer and inner stripes of the outer medulla using qualitative and quantitative transmission electron microscopy. Kidneys of male Sprague-Dawley rats were preserved by in vivo perfusion with glutaraldehyde for light and electron microscopy. The peritubular diameter and cell height were determined by direct measurements on tubule cross sections. Morphometric analyses were performed on montages of tubule cross sections. The peritubular diameter of the TAL was similar in the three regions under investigation, but the TAL cells were taller in the inner stripe than in the inner cortex and outer stripe. Morphometry revealed significant differences between the three regions with respect to the mean tubular cross-sectional area (AT), the surface density (SV), and the surface area per mm of tubule (ST) of apical and basolateral plasma membranes, and the volume density (VV) of mitochondria. The major morphologic division appeared to be between the inner stripe segment and the remainder of the TAL. These findings document the presence of significant morphologic heterogeneity of the rat TAL.
直到最近，尽管有生理和生化方面的相反证据，但大鼠髓袢升支粗段（TAL）一直被认为是一种形态学上均一的结构。本研究旨在使用定性和定量透射电子显微镜检查内皮质以及外髓质外带和内带中TAL的超微结构特征。通过用戊二醛进行体内灌注来保存雄性Sprague-Dawley大鼠的肾脏，用于光学显微镜和电子显微镜观察。通过对肾小管横截面进行直接测量来确定肾小管周围直径和细胞高度。对肾小管横截面的蒙片进行形态计量分析。在所研究的三个区域中，TAL的肾小管周围直径相似，但内带中的TAL细胞比内皮质和外带中的更高。形态计量学显示，在三个区域之间，关于平均肾小管横截面积（AT）、表面密度（SV）、顶端和基底外侧质膜每毫米肾小管的表面积（ST）以及线粒体的体积密度（VV）存在显著差异。主要的形态学划分似乎存在于内带段和TAL的其余部分之间。这些发现证明了大鼠TAL存在显著的形态学异质性。
