Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
School of Health Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
Int J Biol Macromol. 2020 Jul 15;155:305-314. doi: 10.1016/j.ijbiomac.2020.03.229. Epub 2020 Mar 30.
T cell receptor (TCR)-like antibodies, obtained with the use of phage display technology, sandwich the best of the both arms of the adaptive immune system. In this study, in vitro selections against the latency associated Mycobacterium tuberculosis (Mtb) heat shock protein 16 kDa antigen (16 kDa) presented by HLA-A011 and HLA-A24 were carried out with the use of a human domain phage antibody library. TCR-like domain antibodies (A11Ab and A24Ab) were successfully generated recognizing 16 kDa epitopes presented by HLA-A011 and HLA-A24 molecules respectively. Both antibodies were found to be functional in soluble form and exhibited strong binding capacity against its targets. The results obtained support the future evaluation of these ligands for the development of diagnostic and therapeutic tools for tuberculosis infection.
T 细胞受体 (TCR)-样抗体，利用噬菌体展示技术获得，融合了适应性免疫系统的最佳双臂。在这项研究中，利用人类结构域噬菌体抗体文库针对潜伏相关的结核分枝杆菌（Mtb）热休克蛋白 16 kDa 抗原（16 kDa）进行了针对 HLA-A011 和 HLA-A24 的体外选择。成功生成了分别识别由 HLA-A011 和 HLA-A24 分子呈递的 16 kDa 表位的 TCR 样结构域抗体（A11Ab 和 A24Ab）。两种抗体在可溶性形式下均具有功能，并表现出对其靶标的强烈结合能力。所得结果支持对这些配体进行未来评估，以开发用于结核感染的诊断和治疗工具。
