Faculty of Science and Technology, Seikei University, 3-3-1 Kichijoji-Kitamachi, Musashino-shi, Tokyo 180-8633, Japan.
Corporate Engineering Division, Appliances Company, Panasonic Corporation, Kyoto 619-0237, Japan.
Biointerphases. 2020 Apr 2;15(2):021008. doi: 10.1116/6.0000036.
Matrix effects, which cause a change in ion intensity, occur in mass spectrometry methods including time-of-flight secondary ion mass spectrometry (TOF-SIMS). Matrix effects often cause large issues in quantitative analysis because secondary ions related to a particular molecule could be dramatically enhanced or suppressed regardless of the concentration. To investigate matrix effects in biological samples, the authors evaluated mixed lipid {POPC [1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine, molecular weight (MW) 759.6]}, peptide [leu-enkephalin, neo-leu-enkephalin (amino acid sequence: YAGFL, MW 569.3), and neo-angiotensin II (amino acid sequence: DRVYIHAF, MW 1019.5)] samples. Matrix effect features were investigated by analyzing the concentration dependence of secondary ions in lipid-peptide mixed samples to develop a method that enables quantitative analysis using TOF-SIMS. Matrix effects depended on the lipid-peptide combination. Interestingly, some secondary ions possessed an intensity that was highly dependent on concentration.
基质效应会导致离子强度发生变化，这种现象存在于包括飞行时间二次离子质谱（TOF-SIMS）在内的质谱方法中。基质效应经常会给定量分析带来很大的问题，因为与特定分子相关的二次离子无论浓度如何，都会被显著增强或抑制。为了研究生物样品中的基质效应，作者评估了混合脂质（POPC [1-棕榈酰-2-油酰基-sn-甘油-3-磷酸胆碱，分子量（MW）759.6]）、肽（亮啡肽、新亮啡肽（氨基酸序列：YAGFL，MW 569.3）和新血管紧张素 II（氨基酸序列：DRVYIHAF，MW 1019.5））样品。通过分析脂质-肽混合样品中二次离子的浓度依赖性来研究基质效应特征，从而开发出一种使用 TOF-SIMS 进行定量分析的方法。基质效应取决于脂质-肽的组合。有趣的是，一些二次离子的强度高度依赖于浓度。
