State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, Jiangsu Province, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, Jiangsu Province, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, Jiangsu Province, China.
Carbohydr Polym. 2020 Jun 1;237:116137. doi: 10.1016/j.carbpol.2020.116137. Epub 2020 Mar 9.
Enzymatic modification is generally applied to enhance desirable attributes of starch. In this study, β-cyclodextrin glycosyltransferase (β-CGTase) together with a specific cyclodextrinase (CDase) was used to modify corn starch. For single β-CGTase treatment, the molecular weight (M) was reduced to 36.7 × 10 g/mol and 7.7 × 10 g/mol for 1 h and 6 h reaction, and the debranched samples had a high proportion of chains with degree of polymerization (DP) < 13, indicating an apparent depolymerization effect. CDase could hardly act on starch, while it had a high preference for cyclodextrin hydrolysis and produced mainly maltotetraose with β-CGTase. There was a synergistic effect between β-CGTase and CDase in starch degradation. Interestingly, the utilization of dual-enzyme significantly promoted the retrogradation inhibition property of starch, reducing the retrogradation enthalpy from 5.65 J/g to 1.42 J/g. It was proved that β-CGTase and CDase had a great prospect in the future application.
酶法修饰通常用于增强淀粉的理想特性。在这项研究中，β-环糊精葡萄糖基转移酶（β-CGTase）与特定的环糊精酶（CDase）一起用于修饰玉米淀粉。在单一β-CGTase 处理下，1 h 和 6 h 反应后，分子量（M）分别降低至 36.7×10^3g/mol 和 7.7×10^3g/mol，支链淀粉的比例较高，聚合度（DP）<13，表明明显的解聚效果。CDase 几乎不能作用于淀粉，而对环糊精水解有较高的偏好，主要产生麦芽四糖与β-CGTase。β-CGTase 和 CDase 在淀粉降解中有协同作用。有趣的是，双酶的利用显著促进了淀粉的回生抑制性能，使回生焓从 5.65 J/g 降低到 1.42 J/g。证明β-CGTase 和 CDase 在未来的应用中有很大的前景。
Zotero 插件