Virgili M, Barnabei O, Contestabile A
Department of Biology, University of Bologna, Italy.
Brain Res. 1988 Sep 20;460(2):361-5. doi: 10.1016/0006-8993(88)90382-4.
Methylazoxymethanol acetate (MAM) was injected to female rats at the beginning of the 17th day of gestation. Resulting offspring showed a remarkable decrease in the size of the medial habenula while the interpeduncular nucleus, whose neurons are generated before the time of MAM treatment, appeared anatomically unaffected. Choline acetyltransferase was significantly reduced in the habenulae and in the interpeduncular nucleus suggesting that MAM treatment had depleted a portion of the cholinergic neurons of the medial habenula which project to the interpeduncular nucleus. Aromatic amino acid decarboxylase significantly increased in the interpeduncular nucleus, a likely effect of monoaminergic hyperinnervation in response to partial cholinergic deprivation. MAM strategy can be usefully adopted for the study of general aspects of brain development when connected nuclei showing no overlapping in neuronal generation times are involved.
在妊娠第17天开始时，向雌性大鼠注射乙酸甲基偶氮甲醇（MAM）。所产生的后代内侧缰核的大小显著减小，而脚间核在MAM处理之前其神经元就已生成，在解剖学上似乎未受影响。缰核和脚间核中的胆碱乙酰转移酶显著减少，这表明MAM处理使投射到脚间核的内侧缰核的一部分胆碱能神经元减少。芳香族氨基酸脱羧酶在脚间核中显著增加，这可能是对部分胆碱能剥夺的单胺能超神经支配的一种反应。当涉及神经元生成时间不重叠的相连核时，MAM策略可有效地用于研究大脑发育的一般方面。
