Food Additives and Packaging Division, National Institute of Food and Drug Safety Evaluation , Cheongju, Republic of Korea.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2020 Jun;37(6):895-904. doi: 10.1080/19440049.2020.1746840. Epub 2020 Apr 3.
Synthetic colourants are highly regulated due to their correlation with a variety of health hazards. Regulatory services must be able to detect the substances in a cost-effective, efficient, and sensitive manner. LC-UV and LC-MS/MS methods have been developed to simultaneously detect five illegal colourants in foods, such as: citrus red II, diethyl yellow, dimethyl yellow, metanil yellow, and rhodamine B. This method showed good linearity ( > 0.99) and low limits of detection (0.09-0.19 mg kg) and quantitation (0.26-0.58 mg kg). The recoveries at three standard concentration levels ranged between 80.9% and 120%, with relative standard deviations below 12%. The expanded uncertainties determined for the five colourants in three food matrices were 8.2-19.4%. This method was applied to monitor five illegal colourants in imported and domestic beverages, candies, and sauces. None of the five colourants were found in any of the 510 samples. The method is suitable for quantitative analysis of five illegal colourants simultaneously in various foods and can be applied to improve current surveillance and inspection services.
合成色素由于与多种健康危害有关，因此受到高度监管。监管机构必须能够以具有成本效益、高效且灵敏的方式检测这些物质。已经开发出 LC-UV 和 LC-MS/MS 方法来同时检测食品中的五种非法色素，例如：橘红 II、二乙基黄、二甲黄、米妥尔黄和罗丹明 B。该方法显示出良好的线性（>0.99）和低检测限（0.09-0.19 mg kg）和定量限（0.26-0.58 mg kg）。在三个标准浓度水平下的回收率在 80.9%至 120%之间，相对标准偏差低于 12%。在三种食品基质中确定的这五种色素的扩展不确定度为 8.2-19.4%。该方法已应用于监测进口和国产饮料、糖果和酱汁中的五种非法色素。在 510 个样本中均未发现这五种色素。该方法适用于同时定量分析各种食品中的五种非法色素，并可用于改进当前的监测和检查服务。
