Smith T M, Spanos A, Banks G R
Genetics Division, National Institute for Medical Research, London, UK.
Curr Genet. 1988 Nov;14(5):457-60. doi: 10.1007/BF00521269.
A differential hybridisation screen of an Ustilago maydis genomic DNA library was used to identify DNA sequences transcribed at higher levels under growth conditions which induce nitrate reductase activity. After two rounds of screening, four different sequences showed a strongly enhanced hybridisation signal with an induced cDNA probe relative to a repressed cDNA probe. The sequence in plasmid pMH3007 hybridised to a U. maydis RNA transcript of a 4.2 kb. This is identical in size to a transcript, also nitrate inducible, which hybridised to cloned Aspergillus nidulans nitrate reductase gene sequences at a reduced stringency.
利用玉米黑粉菌基因组DNA文库的差异杂交筛选，来鉴定在诱导硝酸还原酶活性的生长条件下转录水平较高的DNA序列。经过两轮筛选，相对于受抑制的cDNA探针，四个不同的序列与诱导的cDNA探针显示出强烈增强的杂交信号。质粒pMH3007中的序列与一个4.2 kb的玉米黑粉菌RNA转录本杂交。其大小与一个转录本相同，该转录本也受硝酸盐诱导，它能以较低的严谨性与克隆的构巢曲霉硝酸还原酶基因序列杂交。
