Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment (Ministry of Education), College of Resource and Environment, Southwest University, Chongqing 400715, PR China; Chongqing Key Laboratory of Agricultural Resources and Environment, Chongqing 400716, PR China.
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment (Ministry of Education), College of Resource and Environment, Southwest University, Chongqing 400715, PR China.
Bioresour Technol. 2020 Jul;307:123249. doi: 10.1016/j.biortech.2020.123249. Epub 2020 Mar 25.
A set of constructed wetlands (CWs) under different biochar addition ratios (0%, 10%, 20%, and 30%) was established to analyze the pollutant removal performance enhancement and nitrous oxide (NO) emission reduction from various angles, including microbial community structure, functional genes and enzyme activity. Results revealed that the average removal efficiencies of ammonium (NH-N) and total nitrogen (TN) were improved by 2.6%-5.2% and 2.5%-7.0%. Meanwhile, NO emissions were reduced by 56.0%-67.5% after biochar addition. Increased nitrogen removal efficiency and decreased NO emissions resulted from the increase of biochar addition ratio. Biochar addition changed the microbial community diversity and similarity. The relative abundance of functional microorganisms such as Nitrosomonas, Nitrospira, Thauera and Pseudomonas, increased due to biochar addition, which promoted the nitrogen cycle and NO emission reduction. High gene copy number and enzyme activity involved in nitrification and denitrification process were obtained in biochar CWs, moderating NO emission.
建立了一组不同生物炭添加比例（0%、10%、20%和 30%）的人工湿地（CWs），从微生物群落结构、功能基因和酶活性等多个角度分析了污染物去除性能的提高和氧化亚氮（NO）排放的减少。结果表明，添加生物炭后，铵（NH-N）和总氮（TN）的平均去除效率提高了 2.6%-5.2%和 2.5%-7.0%。同时，NO 的排放量减少了 56.0%-67.5%。氮去除效率的提高和 NO 排放量的减少是由于生物炭添加比例的增加所致。生物炭的添加改变了微生物群落的多样性和相似性。由于生物炭的添加，增加了硝化菌、反硝化菌、陶厄氏菌和假单胞菌等功能微生物的相对丰度，促进了氮循环和 NO 减排。在生物炭 CWs 中获得了参与硝化和反硝化过程的高基因拷贝数和酶活性，从而调节了 NO 的排放。
