van Huizen F, Strosberg A D, Cynader M S
Department of Ophthalmology, University of British Columbia, Vancouver, Canada.
Brain Res Dev Brain Res. 1988 Dec 1;44(2):296-301. doi: 10.1016/0165-3806(88)90228-3.
A monoclonal antibody against the muscarinic acetylcholine receptor was used to study the distribution of this receptor within the cat visual cortex at the light and electron microscopic level. Immunolabelling was found to be distributed mainly in cell bodies and dendrites in both young and adult cats. The laminar distribution, however, changed during development from cortical layers IV and to a lesser extent II in kittens of 28-42 days of age into one favouring the upper (L I-III) and lower layers (L V and VI) in kittens and cats more then 60 days of age. Electronmicroscopy revealed staining of membranes of cell bodies and dendrites and a great amount of intra-cellular staining of vesicles and internal membranes. Since the receptors are found on cell bodies and dendrites in both kittens and adults this indicates that different populations of cortical cells express these muscarinic receptors at different postnatal ages.
一种抗毒蕈碱型乙酰胆碱受体的单克隆抗体被用于在光学和电子显微镜水平研究该受体在猫视觉皮层中的分布。在幼猫和成年猫中，免疫标记主要分布在细胞体和树突中。然而，层状分布在发育过程中发生了变化，在28 - 42日龄的小猫中，主要分布在皮层IV层，在较小程度上分布在II层，而在60日龄以上的小猫和猫中，分布更倾向于上层（I - III层）和下层（V层和VI层）。电子显微镜显示细胞体和树突膜有染色，并且大量囊泡和内膜有细胞内染色。由于在幼猫和成年猫的细胞体和树突上都发现了受体，这表明不同群体的皮层细胞在出生后的不同年龄表达这些毒蕈碱受体。
