体内成熟和无血清器官培养中成熟的产前和产后小鼠肾脏之间的比较。

Comparison between mouse kidneys of pre- and postnatal ages maturing in vivo and in serum-free organ culture.

Bertrand L, Brière N, Ferrari J

Département d'Anatomie et de biologie cellulaire, Faculté de Médecine, Université de Sherbrooke, Québec, Canada.

Comp Biochem Physiol B. 1988;91(4):763-9. doi: 10.1016/0305-0491(88)90205-2.

Abstract

To evaluate the influence of age, DNA synthesis and brush border hydrolase activities were determined in mouse kidneys maturing in vivo and in serum-free organ culture. 2. DNA synthesis decreased with advancing age. 3. The protein content and leucylnaphthylamidase, maltase, trehalase, alkaline phosphatase and gamma-glutamyltransferase activities increased with aging. 4. The differences due to age were reproduced in kidneys maturing in culture. 5. These results show that age has a significant effect on the parameters determined, but apparently has no influence on the viability of the kidney explants in culture.

摘要

为评估年龄的影响，我们测定了在体内成熟以及在无血清器官培养条件下的小鼠肾脏中的DNA合成和刷状缘水解酶活性。2. DNA合成随年龄增长而下降。3. 蛋白质含量以及亮氨酰萘基酰胺酶、麦芽糖酶、海藻糖酶、碱性磷酸酶和γ-谷氨酰转移酶的活性随衰老而增加。4. 年龄导致的差异在培养中成熟的肾脏中重现。5. 这些结果表明，年龄对所测定的参数有显著影响，但显然对培养中的肾脏外植体的活力没有影响。