牛垂体前叶组织中生物活性和免疫活性促黄体生成素的浓度。

Concentrations of bioactive and immunoactive luteinizing hormone in bovine anterior pituitary tissue.

作者信息

Moss G E, Lemenager R P, Parfet J R, Adams B M, Adams T E

机构信息

Department of Animal Science, University of Wyoming, Laramie 82071.

出版信息

Domest Anim Endocrinol. 1988 Apr;5(2):185-90. doi: 10.1016/0739-7240(88)90019-7.

DOI:10.1016/0739-7240(88)90019-7

PMID:3224519

Abstract

Tissue concentrations of LH as determined by radioimmunoassay (RIA) may not accurately measure actual changes that could occur in biological activity of the hormone. To examine this possibility, pituitary homogenates from 135 beef cows in various physiological states were analyzed for content of LH by both RIA and an in vitro bioassay. The ratio of biological/immunological active concentrations of LH remained constant (.52 +/- .02) even though tissue concentrations of immunoactive LH differed among groups. Tissue concentrations of bioactive LH were linearly related to, and highly correlated with (P less than .001), tissue concentrations of immunoactive LH. These data indicate that only a fraction of the immunoactive LH in the bovine pituitary is biologically active. However, this fraction does not vary with the reproductive status or plane of nutrition.

摘要

通过放射免疫测定法（RIA）测定的促黄体激素（LH）组织浓度可能无法准确测量该激素生物活性中可能发生的实际变化。为了研究这种可能性，对处于不同生理状态的135头肉用母牛的垂体匀浆进行了RIA和体外生物测定，以分析LH的含量。尽管各组间免疫活性LH的组织浓度不同，但LH的生物活性/免疫活性浓度之比保持恒定（0.52±0.02）。生物活性LH的组织浓度与免疫活性LH的组织浓度呈线性相关且高度相关（P＜0.001）。这些数据表明，牛垂体中只有一部分免疫活性LH具有生物活性。然而，这一部分不会随生殖状态或营养水平而变化。