Mehrab Mohseni Mahdieh, Amani Jafar, Fasihi Ramandi Mahdi, Mahjoubi Forouzandeh, Jafaria Mahyat, Salmanian Ali Hatef
Department of Agricultural Biotechnology, National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Iran J Allergy Asthma Immunol. 2019 Oct 23;18(5):511-522. doi: 10.18502/ijaai.v18i5.1921.
Human epithelial growth factor receptor2 (Her2) and polymorphic epithelial mucin (MUC1) are tumor-associated antigens that have been extensively investigated in adenocarcinomas. Generally, each of these molecules was used separately for diagnosis of adenocarcinomas and as an injective vaccines in cancer therapy researches, but not in the chimeric form as an edible immunogen. In this study, Her2, MUC1, and a novel fusion structure were expressed in the seeds and hairy roots of transgenic plants appropriately. The mice groups were immunized either by feeding of transgenic seeds or hairy roots. All immunized groups showed a considerable rise in anti-glycoprotein serum IgG and IgA, and IFNɣ cytokine. However, the animals received chimeric protein showed significant higher immune responses in comparison to ones received one of these immunogen. The results indicated that the oral immunization of an animal model with transgenic plants could effectively elicit immune responses against two major tumor-associated antigens.
人表皮生长因子受体2（Her2）和多形性上皮黏蛋白（MUC1）是在腺癌中已被广泛研究的肿瘤相关抗原。通常，这些分子各自分别用于腺癌的诊断，并在癌症治疗研究中作为注射用疫苗，但并未以嵌合形式作为可食用免疫原。在本研究中，Her2、MUC1和一种新型融合结构在转基因植物的种子和毛状根中得到了适当表达。小鼠组通过喂食转基因种子或毛状根进行免疫。所有免疫组的抗糖蛋白血清IgG、IgA和IFNɣ细胞因子均显著升高。然而，与接受这些免疫原之一的动物相比，接受嵌合蛋白的动物表现出明显更高的免疫反应。结果表明，用转基因植物对动物模型进行口服免疫可有效引发针对两种主要肿瘤相关抗原的免疫反应。
