Nowras Tayebeh, Fereidouni Mohammad, Safari Hamidreza, Naseri Mohsen
Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran.
Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran.
Iran J Allergy Asthma Immunol. 2019 Oct 23;18(5):523-532. doi: 10.18502/ijaai.v18i5.1922.
Multiple sclerosis (MS) is one of the autoimmune diseases that affects the central nervous system (CNS) and causes myelin loss and axonal damage. Recent studies have shown the important role of autoreactive T cells in the pathogenesis of MS. One of the plants in the Astersa family, which has therapeutic benefits is Artemisia dracunculus L. or Tarragon. In this study, the role of aqueous extract of Tarragon in suppressing Th1 and Th17 cell differentiation and ameliorating experimental autoimmune encephalomyelitis (EAE) was investigated. EAE was induced in C57BL/6 female mice by Hook kit MOG35-55/CFA Emulsion PTX and one group was treated with Tarragon at a dose of 500 mg/kg. Mice were euthanized on day 33 post-immunization, spleens were removed for assessing Th1, Th17 and Treg cells by flow cytometry. We provided evidence that Tarragon (500 mg/kg) significantly ameliorated clinical scores of EAE. We did not observe significant alterations in T cell differentiation to Th1, Th17 or Treg in the spleen of mice during EAE. This is the first experimental evidence showing that administration of aqueous extract of Tarragon reduces the severity of EAE, but the protective effect of Tarragon is independent of alteration in T cells in the spleen. These results suggest other mechanisms for the effectiveness of this extract in improving the EAE process.
多发性硬化症（MS）是一种影响中枢神经系统（CNS）并导致髓鞘丢失和轴突损伤的自身免疫性疾病。最近的研究表明自身反应性T细胞在MS发病机制中起重要作用。紫菀科植物中的一种具有治疗益处的植物是龙蒿，即狭叶青蒿。在本研究中，研究了龙蒿水提取物在抑制Th1和Th17细胞分化以及改善实验性自身免疫性脑脊髓炎（EAE）方面的作用。通过Hook试剂盒用MOG35-55/CFA乳剂PTX在C57BL/6雌性小鼠中诱导EAE，一组以500mg/kg的剂量用龙蒿治疗。在免疫后第33天对小鼠实施安乐死，取出脾脏通过流式细胞术评估Th1、Th17和调节性T（Treg）细胞。我们提供的证据表明龙蒿（500mg/kg）显著改善了EAE的临床评分。在EAE期间，我们未观察到小鼠脾脏中T细胞向Th1、Th17或Treg分化的显著变化。这是首个实验证据表明给予龙蒿水提取物可降低EAE的严重程度，但龙蒿的保护作用与脾脏中T细胞的改变无关。这些结果提示了该提取物改善EAE进程有效性的其他机制。
