Bocchini V, Artault J C, Rebel G, Dreyfus H, Massarelli R
Istituto di Biologia Cellulare, Universita di Perugia, Italia.
Dev Neurosci. 1988;10(4):270-6. doi: 10.1159/000111977.
Cultures of microglial cells were prepared from primary rat brain cultures of astrocytes. Microglia appeared under two main forms: ameboid and ramified. Only the former were capable of ingesting opsonized latex particles of 1 and 6 micron; while the latter, which appeared later in culture, were not capable of phagocytosis. When gangliosides (10(-6) M), which are known for their neuronotrophic capacity in vitro, were added to the growth medium (for 20 min) they stimulated the phagocytosis of ameboid microglia 2-fold.
小胶质细胞培养物取自原代大鼠星形胶质细胞脑培养物。小胶质细胞呈现两种主要形态：阿米巴样和分枝状。只有前者能够吞噬1微米和6微米的调理乳胶颗粒；而后者在培养后期出现，不具备吞噬能力。当具有体外神经营养能力的神经节苷脂（10^(-6) M）添加到生长培养基中（20分钟）时，它们会使阿米巴样小胶质细胞的吞噬作用增强两倍。
