Laboratory of Nano-Bioengineering, National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), Moscow, Russian Federation.
Department of Clinical Immunology and Allergology, Sechenov First Moscow State Medical University, Moscow, Russian Federation.
Methods Mol Biol. 2020;2135:225-236. doi: 10.1007/978-1-0716-0463-2_13.
A quantum dot (QD)-based lab-on-bead system is a unique tool for multiple analysis of cancer markers in human serum samples by using a flow cytometer. In terms of specificity and sensitivity, this method is comparable with ELISA, the "gold standard" of serological in-clinic detection of single analytes. Fluorescent microspheres encoded with QDs have been used for the quantitative detection of free and total prostate-specific antigen in human serum samples. Developed multiplex assay demonstrates a clear discrimination between serum samples from control subjects and cancer patients. The proposed QD-based method is adaptable and makes it possible to develop numerous clinical tests with decreased duration and cost for early diagnosis of various diseases.
基于量子点的微珠上实验室系统是一种独特的工具，可通过流式细胞仪对人血清样本中的癌症标志物进行多种分析。就特异性和灵敏度而言，该方法可与 ELISA（单分析物血清临床检测的“金标准”）相媲美。用量子点编码的荧光微球已被用于定量检测人血清样本中的游离和总前列腺特异性抗原。开发的多重分析方法可清楚地区分对照受试者和癌症患者的血清样本。所提出的基于量子点的方法具有适应性，可以开发出许多具有较短时间和较低成本的临床检测方法，用于各种疾病的早期诊断。
