North-Western State Medical University named after I.I. Mechnikov, Saint Petersburg, Russia.
Mycoses. 2020 Jun;63(6):596-603. doi: 10.1111/myc.13083. Epub 2020 Apr 21.
Allergic bronchopulmonary aspergillosis (ABPA) is a lung disease in patients with asthma or cystic fibrosis (CF) caused by chronic allergic inflammation to Aspergillus spp. antigens. The role of different immunological mediators in the formation of chronic allergic inflammation in patients with ABPA is not sufficiently explored.
This study aimed to investigate serum levels of thymic stromal lymphopoietin (TSLP), thymus and activated chemokine (TARC) as well as IL-8 in patients with ABPA, and to evaluate their diagnostic and monitoring value in the disease.
PATIENTS/METHODS: Prospective study included 21 patients with ABPA, 25 patients with severe asthma with fungal sensitisation (SAFS), 37 patients with severe asthma without fungal sensitisation (SAwFS), and 16 healthy people. In patients with ABPA, the serum levels of biomarkers were determined at baseline and after 12 weeks of itraconazole therapy. Serum levels of total IgE, Aspergillus-fumigatus-specific IgE, TSLP, TARC, IL-8 were analysed by enzyme-linked immunosorbent assay.
In patients with ABPA we established significantly higher serum levels of TARC, IL-8, total IgE, Aspergillus-fumigatus-specific IgE and peripheral blood eosinophil counts, compared to patients with SAwFS. There were no differences in TSLP levels between the examined groups of patients. Serum TARC levels were positively correlated to serum total IgE levels, A fumigatus-specific IgE levels and peripheral blood eosinophil counts and also negatively correlated to lung function (FEV ). Longitudinally, serum levels TARC, total IgE and peripheral blood eosinophil counts significant decreased after treatment of ABPA.
Thymus and activated chemokine is a useful test in diagnosing and monitoring response to the antifungal treatment of patients with ABPA.
变应性支气管肺曲霉病（ABPA）是一种由曲霉菌属抗原引起的哮喘或囊性纤维化（CF）患者的肺部疾病，其特征为慢性过敏炎症。不同免疫介质在 ABPA 患者慢性过敏炎症形成中的作用尚未得到充分探索。
本研究旨在探讨变应性支气管肺曲霉病（ABPA）患者血清胸腺基质淋巴细胞生成素（TSLP）、胸腺激活调节趋化因子（TARC）和白细胞介素-8（IL-8）的水平，并评估其在疾病诊断和监测中的价值。
患者/方法：前瞻性研究纳入了 21 例 ABPA 患者、25 例有真菌致敏的严重哮喘（SAFS）患者、37 例无真菌致敏的严重哮喘（SAwFS）患者和 16 例健康人。在 ABPA 患者中，分别在基线时和伊曲康唑治疗 12 周后测定生物标志物的血清水平。采用酶联免疫吸附试验分析总 IgE、烟曲霉特异性 IgE、TSLP、TARC、IL-8 的血清水平。
与 SAFwFS 患者相比，ABPA 患者的 TARC、IL-8、总 IgE、烟曲霉特异性 IgE 和外周血嗜酸性粒细胞计数显著升高。各组间 TSLP 水平无差异。血清 TARC 水平与总 IgE 水平、A 烟曲霉特异性 IgE 水平和外周血嗜酸性粒细胞计数呈正相关，与肺功能（FEV1）呈负相关。ABPA 患者经治疗后，血清 TARC、总 IgE 和外周血嗜酸性粒细胞计数均显著下降。
胸腺激活调节趋化因子是诊断和监测 ABPA 患者抗真菌治疗反应的有用指标。
