采用针对 wciP 基因的 PCR 和 BsmAI 限制组合来确定血清型 6A、6B、6C 和 6D 肺炎链球菌。
The use of PCR and BsmAI restriction combination targeting wciP gene to determine serotype 6A, 6B, 6C and 6D Streptococcus pneumoniae.
机构信息
Molecular Bacteriology Laboratory, Eijkman Institute for Molecular Biology, Indonesia.
School of Life Sciences and Technology (SITH), Bandung Institute of Technology (ITB), Indonesia.
出版信息
J Microbiol Methods. 2020 May;172:105909. doi: 10.1016/j.mimet.2020.105909. Epub 2020 Apr 1.
PMID:32246952
Abstract
We designed a sensitive and reliable method to distinguish serogroup 6 using PCR followed by enzymatic restriction digest. We discovered that this serotyping method was able to distinguish serotypes 6A, 6B, 6C, and 6D based on the recognition site of BsmAI in the wciP region.
摘要
我们设计了一种灵敏可靠的方法，使用 PCR 结合酶切反应来区分血清群 6。我们发现，这种血清分型方法能够根据 wciP 区域中 BsmAI 的识别位点区分血清型 6A、6B、6C 和 6D。
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