Fay T N, Jacobs I, Teisner B, Poulsen O, Chapman M G, Stabile I, Bohn H, Westergaard J G, Grudzinskas J G
Joint Academic Unit of Obstetrics, Gynaecology and Reproductive Physiology, London Hospital, U.K.
Eur J Obstet Gynecol Reprod Biol. 1988 Sep;29(1):73-85. doi: 10.1016/0028-2243(88)90167-0.
Rabbit antihuman antibodies were derived by the injection of fractions of second trimester amniotic fluid known to contain proteins of endometrial/decidual origin. Using standard separation and absorption procedures, two antibody preparations were generated which demonstrated specificities against two and three proteins, respectively, in line immunoelectrophoresis and crossed immunoelectrophoresis. Analysis against proteins of fetal, maternal, endometrial and placental origin revealed that the bispecific antiserum reacted only with placental protein 14 (PP14; also known as progestagen-dependent endometrial protein, PEP) and one other hitherto undescribed antigen referred to as Fetal Antigen 1 (FA-1) molecular mass 60 kDa; electrophoretic mobility: slow; alpha 1-alpha 2; fast, albumin. The trispecific antiserum demonstrated specifities against placental protein 12 (PP12), alpha-fetoprotein (AFP) and another previously undescribed antigen referred to as Fetal Antigen 2 (FA-2) molecular mass 35 and 140 kDa; electrophoretic mobility: albumin. Following purification, monospecific antisera against each of these proteins (with the exception of AFP) were derived in new rabbits. Maternal and fetal blood, amniotic fluid and aqueous extracts from endometrial/decidual and placental tissues were analysed in rocket immunoelectrophoresis using these antisera to examine the distribution in these tissues. The analyses demonstrated a pattern of distribution typical for proteins of endometrial/decidual origin in these compartments in the case of PP12 and PP14, but suggested that the primary source of origin of FA-1 and FA-2 may be the fetus.
兔抗人抗体是通过注射已知含有子宫内膜/蜕膜来源蛋白质的孕中期羊水组分获得的。采用标准的分离和吸收程序，制备了两种抗体制剂，它们在直线免疫电泳和交叉免疫电泳中分别对两种和三种蛋白质表现出特异性。对胎儿、母体、子宫内膜和胎盘来源的蛋白质进行分析发现，双特异性抗血清仅与胎盘蛋白14（PP14；也称为孕激素依赖性子宫内膜蛋白，PEP）和另一种迄今未描述的抗原发生反应，该抗原称为胎儿抗原1（FA-1），分子量60 kDa；电泳迁移率：慢；α1-α2；快，白蛋白。三特异性抗血清对胎盘蛋白12（PP12）、甲胎蛋白（AFP）和另一种先前未描述的抗原表现出特异性，该抗原称为胎儿抗原2（FA-2），分子量35和140 kDa；电泳迁移率：白蛋白。纯化后，在新的兔子中获得了针对这些蛋白质中每一种（除AFP外）的单特异性抗血清。使用这些抗血清通过火箭免疫电泳分析母体和胎儿血液、羊水以及子宫内膜/蜕膜和胎盘组织的水提取物，以检查这些组织中的分布情况。分析表明，在PP12和PP14的情况下，这些隔室中这些蛋白质具有典型的子宫内膜/蜕膜来源的分布模式，但表明FA-1和FA-2的主要来源可能是胎儿。
