使用靶标引发的锁式探针分析法对固定细胞和组织中的单个微小RNA分子进行可视化分析。

Visualization of individual microRNA molecules in fixed cells and tissues using target-primed padlock probe assay.

作者信息

Lin Chen, Jiang Meng, Duan Shanshan, Qiu Jianlong, Hong Yujuan, Wang Xin, Chen Xiaoyuan, Ke Rongqin

机构信息

School of Medicine, Huaqiao University, Quanzhou, Fujian, China.

Department of Pathology, 910th Hospital of the Joint Logistics Support Force of PLA, Quanzhou, Fujian, China.

出版信息

Biochem Biophys Res Commun. 2020 Jun 4;526(3):607-611. doi: 10.1016/j.bbrc.2020.03.134. Epub 2020 Apr 2.

DOI:10.1016/j.bbrc.2020.03.134

PMID:32247612

Abstract

MicroRNAs (miRNAs) are key regulators of gene expression at the posttranscriptional level. Precisely profiling of miRNA expression will help us to better understand their roles in normal and diseased cells and tissues. Here we describe in situ miRNA detection by padlock probing and miRNA target-primed rolling circle amplification. We optimized our protocol and showed it can be applied to both fixed cells and tissue sections. The method can be used in basic research and potentially in clinical diagnostics in the future.

摘要

微小RNA（miRNA）是转录后水平基因表达的关键调节因子。精确分析miRNA表达将有助于我们更好地理解它们在正常及病变细胞和组织中的作用。在此，我们描述了通过锁式探针和miRNA靶标引发的滚环扩增进行原位miRNA检测。我们优化了实验方案，并证明其可应用于固定细胞和组织切片。该方法可用于基础研究，未来还有望应用于临床诊断。