Laboratory of Genetics, NAIK-Research Institute for Animal Breeding, Nutrition and Meat Science, Gesztenyés u. 1., 2053 Herceghalom, Hungary.
Department of Animal Breeding and Genetics, University of Veterinary Medicine, István u. 2, 1078, Budapest, Hungary.
Animal. 2020 Sep;14(9):1786-1792. doi: 10.1017/S1751731120000634. Epub 2020 Apr 6.
Hungarian Grey is an indigenous cattle breed that is one of the national symbols of Hungary. However, genetic description of the Hungarian Grey cattle has not yet been conducted based on whole-genome screening. Using the GeneSeek high-density Bovine SNP (single nucleotide polymorphism) 150 K BeadChip, we sampled the genome of 36 Hungarian Grey, 12 Maremmana, 13 Hungarian Fleckvieh and 5 Holstein-Friesian cattle for population studies and used data of 139 other cattle from an additional dataset created on European cattle breeds (Upadhyay et al.2017. Heredity 118, 169-176). The performance of a multidimensional scaling plot showed that Hungarian Grey clustered independently from other European cattle. The number and total length of runs of homozygosity (ROH) is similar or slightly below the value of other European cattle; FROH coefficients (proportion of the autosomal genome covered by ROH) are similar to Maremmana and Maronesa. The frequency of ROH does not show increased values as it can be noticed in Heck and Maltese. These results indicate that the Hungarian Grey cattle have been successfully maintained avoiding negative genetic effects, and reflect the uniqueness among European cattle. The identification of breed-specific loci has been aimed at differentiating Hungarian Grey (n = 136 in this case) from other cattle breeds (n = 169). Ten loci (-log10P > 5) were identified as markers capable for differentiation of Hungarian Grey. These markers are located on chromosomes 6, 14, 15, 16, 20 and 24.
匈牙利灰牛是匈牙利的本土牛种之一，也是匈牙利的国家象征之一。然而，目前尚未对匈牙利灰牛进行基于全基因组筛选的遗传描述。本研究使用 GeneSeek 高密度牛 SNP（单核苷酸多态性）150K 珠芯片，对 36 头匈牙利灰牛、12 头马雷马牛、13 头匈牙利红牛和 5 头荷斯坦弗里生牛进行了基因组采样，用于群体研究，并利用来自欧洲牛种的额外数据集（Upadhyay 等人，2017 年。遗传 118，169-176）中的 139 头其他牛的数据。多维尺度图的表现表明，匈牙利灰牛与其他欧洲牛种独立聚类。纯合子连续（ROH）的数量和总长度与其他欧洲牛种相似或略低；FROH 系数（ROH 覆盖的常染色体基因组比例）与马雷马牛和马罗内萨牛相似。ROH 的频率没有像在赫克和马耳他人中那样出现增加的值。这些结果表明，匈牙利灰牛的成功保种避免了负面的遗传影响，反映了其在欧洲牛种中的独特性。鉴定特定品种的基因座旨在将匈牙利灰牛（本例中为 136 头）与其他牛种（169 头）区分开来。已确定 10 个基因座（-log10P > 5）作为能够区分匈牙利灰牛的标记。这些标记位于 6、14、15、16、20 和 24 号染色体上。
