College of Food Science, Shenyang Agricultural University, Shenyang, Liaoning, 110866, China.
College of Chemistry and Life Science, Anshan Normal College, Anshan, Liaoning, 114007, China.
J Food Sci. 2020 Apr;85(4):1307-1318. doi: 10.1111/1750-3841.15109. Epub 2020 Apr 6.
We aimed to investigate) the effects of Aronia melanocarpa berry extract (AMBE) on hepatic insulin resistance and its mechanism at the molecular level in high-fat diet (HFD)- and streptozotocin (STZ)-induced type 2 diabetes mellitus (T2DM) rats. The rats were supplemented with AMBE at doses of 100 and 400 mg/kg body weight (bw) daily for 8 weeks. AMBE significantly reduced blood glucose and serum insulin levels and the homeostatic model assessment for insulin resistance score; improved glucose tolerance; increased hepatic glycogen content; and regulated glucose metabolism enzyme activity, including glucokinase, pyruvate kinase, phosphoenolpyruvate carboxykinase, and glucose-6-phosphatase in the liver. AMBE also reduced lipid accumulation and oxidative stress along with inflammation in the hepatic tissue of T2DM rats and improved hepatic function. The phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway was activated by AMBE through the elevation of insulin receptor substrate-2, PI3K, Akt, and glycogen synthase kinase-3β phosphorylation and glucose transporter 2, which might contribute to the promotion of glycogen synthesis and improvement of hepatic insulin resistance. AMBE shows promise as an ingredient of functional foods for alleviating hepatic insulin resistance in T2DM. PRACTICAL APPLICATION: The extract from the berries of Aronia melanocarpa (Michx.) Elliott (AMBE), with its relatively high content of polyphenolic compounds, has been shown to exert hypoglycemic effects in animal models of diabetes. Our findings support the use of A. melanocarpa as a functional food additive for the alleviation of hepatic insulin resistance and the management of glucose homeostasis in T2DM.
我们旨在研究黑果腺肋花楸果提取物（AMBE）对高脂肪饮食（HFD）和链脲佐菌素（STZ）诱导的 2 型糖尿病（T2DM）大鼠肝胰岛素抵抗的影响及其在分子水平上的机制。大鼠每天补充 AMBE 的剂量为 100 和 400mg/kg 体重（bw），共 8 周。AMBE 可显著降低血糖和血清胰岛素水平以及稳态模型评估的胰岛素抵抗评分；改善葡萄糖耐量；增加肝糖原含量；调节葡萄糖代谢酶活性，包括肝中的葡糖激酶、丙酮酸激酶、磷酸烯醇丙酮酸羧激酶和葡萄糖-6-磷酸酶。AMBE 还可减少 T2DM 大鼠肝组织中的脂质积累和氧化应激以及炎症，并改善肝功能。PI3K/蛋白激酶 B（Akt）信号通路通过升高胰岛素受体底物-2、PI3K、Akt 和糖原合酶激酶-3β的磷酸化以及葡萄糖转运蛋白 2 被 AMBE 激活，这可能有助于促进糖原合成和改善肝胰岛素抵抗。AMBE 有望成为缓解 T2DM 肝胰岛素抵抗的功能性食品的成分。实际应用：黑果腺肋花楸（Michx.）Elliott（AMBE）浆果的提取物，由于其含有较高含量的多酚化合物，已被证明在糖尿病动物模型中具有降血糖作用。我们的研究结果支持将黑果腺肋花楸作为功能性食品添加剂，用于缓解 T2DM 肝胰岛素抵抗和葡萄糖稳态的管理。
