Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China.
J Invertebr Pathol. 2020 Jun;173:107367. doi: 10.1016/j.jip.2020.107367. Epub 2020 Apr 3.
Decapod iridescent virus 1 (DIV1) was proven to be the aetiological agent of a disease causing mass die-offs of shrimp, prawn and crayfish. The specific purpose of this study was to develop a new sensitive real-time PCR method for the specific detection of DIV1. A pair of primers that amplify a 142 bp fragment and a TaqMan probe were selected for the major capsid protein gene of DIV1. They were shown to be specific for DIV1 and did not react with other common shrimp pathogens or healthy shrimp DNA. The method could detect as virus levels as low as 1.2 copies of DIV1 plasmid DNA.
十足目虹彩病毒 1 型（DIV1）已被证实是导致虾、对虾和小龙虾大量死亡的病原体。本研究的目的是开发一种新的敏感实时 PCR 方法，用于特异性检测 DIV1。选择一对引物扩增 142bp 片段和 TaqMan 探针，用于 DIV1 的主要衣壳蛋白基因。结果表明，该方法具有特异性，不与其他常见虾类病原体或健康虾类 DNA 发生反应。该方法可检测到低至 1.2 个拷贝的 DIV1 质粒 DNA 的病毒水平。
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