维生素 D 代谢物影响胰岛素分泌β细胞（INS1E）中与细胞活力和功能相关基因的表达。

Vitamin D metabolites influence expression of genes concerning cellular viability and function in insulin producing β-cells (INS1E).

机构信息

Hormone Laboratory, Dep. of Medical Biochemistry, Oslo University Hospital, Norway; Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Norway.

Hormone Laboratory, Dep. of Medical Biochemistry, Oslo University Hospital, Norway.

出版信息

Gene. 2020 Jul 1;746:144649. doi: 10.1016/j.gene.2020.144649. Epub 2020 Apr 3.

DOI:10.1016/j.gene.2020.144649

PMID:32251702

Abstract

BACKGROUND

Studies have shown that vitamin D can enhance glucose-stimulated insulin secretion (GSIS) and change the expression of genes in pancreatic β-cells. Still the mechanisms linking vitamin D and GSIS are unknown.

MATERIAL AND METHODS

We used an established β-cell line, INS1E. INS1E cells were pre-treated with 10 nM 1,25(OH)vitamin D or 10 nM 25(OH)vitamin D for 72 h and stimulated with 22 mM glucose for 60 min. RNA was extracted for gene expression analysis.

RESULTS

Expression of genes affecting viability, apoptosis and GSIS changed after pre-treatment with both 1,25(OH)vitamin D and 25(OH)vitamin D in INS1E cells. Stimulation with glucose after pre-treatment of INS1E cells with 1,25(OH)vitamin D resulted in 181 differentially expressed genes, whereas 526 genes were differentially expressed after pre-treatment with 25(OH)vitamin D.

CONCLUSION

Vitamin D metabolites may affect pancreatic β-cells and GSIS through changed gene expression for genes involved in β-cell function and viability.

摘要

背景

研究表明，维生素 D 可以增强葡萄糖刺激的胰岛素分泌（GSIS）并改变胰岛 β 细胞中基因的表达。然而，维生素 D 与 GSIS 之间的联系机制尚不清楚。

材料与方法

我们使用了一种已建立的 β 细胞系 INS1E。INS1E 细胞先用 10 nM 1,25（OH）维生素 D 或 10 nM 25（OH）维生素 D 预处理 72 h，然后用 22 mM 葡萄糖刺激 60 min。提取 RNA 进行基因表达分析。

结果

用 1,25（OH）维生素 D 和 25（OH）维生素 D 预处理 INS1E 细胞后，影响细胞活力、凋亡和 GSIS 的基因表达发生了变化。用 1,25（OH）维生素 D 预处理 INS1E 细胞后，用葡萄糖刺激导致 181 个差异表达基因，而用 25（OH）维生素 D 预处理后则有 526 个差异表达基因。