Bartol F F, Wiley A A, Coleman D A, Wolfe D F, Riddell M G
Auburn University, AL 36849.
J Anim Sci. 1988 Nov;66(11):3000-9. doi: 10.2527/jas1988.66113000x.
To determine effects of age and administration and withdrawal of a synthetic progestin (P) on endometrial development and DNA synthesis, ewe lambs were ovariectomized on d 0 (birth) and assigned to one of four groups (n = three/group) that provided (by means of hemihysterectomy) the following uterine tissue types: 1) d 0 control, 2) d 13 control, 3) d 26 control, 4) d 13 after 13 d exposure to P (13P) and 5) d 26 after P exposure from d 0 to 13 (26P). Uterine tissues were processed for histology or explanted with [methyl-3 H] thymidine for autoradiography. Labeling index (LI) was determined for stroma and epithelium in caruncular and intercaruncular endometrial areas and for lumenal and glandular epithelium in uteri with glands. Endometrial glands were absent on d 0, evident at d 13 and well developed by d 26. Day 0 LI was greater (P less than .05) in caruncular than in intercaruncular areas, and greater in stromal than in epithelial tissues. Relationships were reversed in d 13 endometrium (day X endometrial area, P less than .07). Caruncular stromal LI was greater on d 0 than later (P less than .02), whereas intercaruncular epithelial LI was greater after d 0 (P less than .05), but decreased from d 13 to 26 (P less than .05). Glandular epithelial LI was higher on d 13 than on d 26 (P less than .03). Administration of P inhibited endometrial gland development and suppressed d 13P intercaruncular LI (P less than .05). Withdrawal of P was followed by endometrial gland development and increased (P less than .01) intercaruncular epithelial LI in d 26P uteri. Ovary-independent initiation of endometrial gland development involves age- and region-specific alterations in DNA synthesis and could involve negative control.
为了确定年龄以及合成孕激素（P）的给药和撤药对子宫内膜发育及DNA合成的影响，在第0天（出生时）对母羊羔羊进行卵巢切除，并将其分为四组（每组n = 3只），通过半子宫切除术提供以下子宫组织类型：1）第0天对照组，2）第13天对照组，3）第26天对照组，4）在暴露于P 13天后的第13天（13P），5）从第0天至第13天暴露于P后的第26天（26P）。对子宫组织进行组织学处理，或用[甲基-³H]胸苷进行外植以进行放射自显影。测定肉阜和肉阜间子宫内膜区域的基质和上皮以及有腺体子宫的腔上皮和腺上皮的标记指数（LI）。第0天时无子宫内膜腺体，第13天时明显可见，到第26天时发育良好。第0天肉阜处的LI高于肉阜间区域（P < 0.05），基质中的LI高于上皮组织。在第13天的子宫内膜中这种关系相反（第X天子宫内膜区域，P < 0.07）。肉阜基质LI在第0天比之后更高（P < 0.02），而肉阜间上皮LI在第0天后更高（P < 0.05），但从第13天到第26天降低（P < 0.05）。腺上皮LI在第13天高于第26天（P < 0.03）。给予P抑制了子宫内膜腺体发育并抑制了13P肉阜间LI（P < 0.05）。撤掉P后，26P子宫中出现子宫内膜腺体发育且肉阜间上皮LI增加（P < 0.01）。不依赖卵巢的子宫内膜腺体发育起始涉及DNA合成中年龄和区域特异性的改变，并且可能涉及负调控。
