Senger P L, Becker W C, Davidge S T, Hillers J K, Reeves J J
Washington State University, Pullman 99164-6332.
J Anim Sci. 1988 Nov;66(11):3010-16. doi: 10.2527/jas1988.66113010x.
The objective of this study was to compare conception to artificial insemination (AI) services in dairy cattle when semen was deposited into the uterine body or into both uterine horns (cornual insemination). Nine herdsman inseminators (HI) in four commercial dairy herds in Washington constituted the experimental units. Herds ranged in size from 393 cows to 964 cows. The duration of the experiment was 12 mo in three herds and 18 mo in the fourth herd. At the beginning of the experiment all inseminators were trained to deposit semen in the body of the uterus. Inseminators were instructed to use this method for 6 mo. Following employment of body deposition, the same inseminators were retrained to deposit one-half of the semen into the right uterine horn and one-half into the left uterine horn. Cornual inseminations were performed for 6 mo. A total of 4,178 services constituted the data set. Milk samples were collected from cows on the day of insemination and later were assayed for progesterone (P4). There was variation (P less than .01) in conception associated with month of insemination and insemination method (P less than .001). The monthly variation was not associated with season of the year. Least squares means for conception when semen was deposited in the uterine body was 44.7%, compared with 64.6% when cornual insemination was employed. The insemination treatment X inseminator interaction was not significant. Results suggest that cornual insemination provides an alternative to deposition of semen in the uterine body.
本研究的目的是比较在奶牛人工授精(AI)服务中,将精液注入子宫体或双侧子宫角(子宫角输精)时的受胎情况。华盛顿州四个商业化奶牛场的九名牧民输精员(HI)构成了实验单位。牛群规模从393头奶牛到964头奶牛不等。三个牛群的实验持续时间为12个月,第四个牛群为18个月。实验开始时,所有输精员都接受了将精液注入子宫体的培训。输精员被要求使用这种方法6个月。在采用子宫体输精后,同样的输精员又接受了再培训,将一半精液注入右子宫角,另一半注入左子宫角。子宫角输精进行了6个月。共有4178次输精服务构成了数据集。在输精当天从奶牛采集牛奶样本,随后检测孕酮(P4)。受胎情况与输精月份和输精方法有关(P <.01和P <.001),存在差异。每月的差异与一年中的季节无关。精液注入子宫体时的最小二乘均值受胎率为44.7%,而采用子宫角输精时为64.6%。输精处理×输精员的交互作用不显著。结果表明,子宫角输精为精液注入子宫体提供了一种替代方法。