犬瘟热病毒诱导犬脑组织中 GABAGABA 和 GAT1 表达下调。

Canine distemper virus induces downregulation of GABAGABA, and GAT1 expression in brain tissue of dogs.

机构信息

Department of Pathology, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Turkey.

Department of Genetic, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Turkey.

出版信息

Arch Virol. 2020 Jun;165(6):1321-1331. doi: 10.1007/s00705-020-04617-3. Epub 2020 Apr 6.

DOI:10.1007/s00705-020-04617-3

PMID:32253618

Abstract

The aim of the study was to determine the expression profiles of GABA, GABA, and GAT1 using RT-PCR and the immunoreactivity of GAT1 via immunohistochemical and immunofluorescence assays in CDV-infected brain tissue of dogs. For this purpose, dogs with CDV and dogs without CDV were selected. The mRNA transcript levels of GABA, GABA, and GAT1 were significantly downregulated in brain tissue in the CDV-infected group as compared with that in non-CDV-infected brain tissue in the control group (p < 0.01, p < 0.001). In addition, the immunoreactivity of GAT1 in CDV-infected brain tissue was significantly lower than in the uninfected group (p < 0.05). We conclude that one of the main causes of myoclonus in CDV infections may be the blockage of postsynaptic inhibition in neurons or a lack of metabolism of GABA. In addition, a GABA neurotransmission imbalance could play a role in demyelination in CDV infections.

摘要

本研究旨在通过 RT-PCR 确定 GABA、GABA 和 GAT1 的表达谱，并通过免疫组织化学和免疫荧光检测 CDV 感染犬脑组织中 GAT1 的免疫反应性。为此，选择了患有 CDV 和未患有 CDV 的犬。与对照组中非 CDV 感染脑组织相比，CDV 感染组脑组织中 GABA、GABA 和 GAT1 的 mRNA 转录水平显著下调（p<0.01，p<0.001）。此外，CDV 感染脑组织中 GAT1 的免疫反应性明显低于未感染组（p<0.05）。我们得出结论，CDV 感染中肌阵挛的一个主要原因可能是神经元突触后抑制的阻断或 GABA 代谢的缺乏。此外，GABA 神经递质传递失衡可能在 CDV 感染中的脱髓鞘中起作用。