人脐带间充质干细胞：角膜上皮再生的有前途策略。
Human umbilical cord-mesenchymal stem cells: a promising strategy for corneal epithelial regeneration.
机构信息
Regenerative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, SAINS@BERTAM, Kepala Batas, Penang 13200, Malaysia.
Faculty of Medical Laboratory Sciences, National University Sudan, Khartoum, Sudan.
出版信息
Regen Med. 2020 Mar;15(3):1381-1397. doi: 10.2217/rme-2019-0103. Epub 2020 Apr 7.
As a strategy to improve the outcome of cultivated corneal epithelial transplantation, the role of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) is investigated in promoting corneal epithelial growth and functions. Human telomerase-immortalized corneal epithelial cells were characterized and its functions evaluated by scratch migration assay, cellular senescence, HLA expression and spheres formation with hUC-MSC. Expression of corneal epithelial markers was influenced by the duration and method of co-culture. Indirect co-culture improved cellular migration and delayed senescence when treated after 3 and 5 days. hUC-MSC downregulated expression of HLA Class I and II in IFN-γ-stimulated human telomerase-immortalized corneal epithelial cells. hUC-MSC promote corneal epithelial growth and functions after treatment with hUC-MSC.
为了提高培养角膜上皮移植的效果，研究了人脐带间充质干细胞（hUC-MSCs）在促进角膜上皮生长和功能方面的作用。用人端粒酶永生化角膜上皮细胞进行特征描述，并通过划痕迁移试验、细胞衰老、HLA 表达和与 hUC-MSC 形成球体来评估其功能。共培养的持续时间和方法影响角膜上皮标志物的表达。间接共培养可改善细胞迁移，在处理后第 3 天和第 5 天可延迟衰老。hUC-MSC 下调 IFN-γ刺激的人端粒酶永生化角膜上皮细胞中 HLA Ⅰ类和Ⅱ类的表达。hUC-MSC 在经 hUC-MSC 处理后可促进角膜上皮的生长和功能。
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