Fukui Yuuka, Fukuda Megumi, Fujimoto Keiji
The Center for Chemical Biology, School of Fundamental Science and Technology, Graduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
J Mater Chem B. 2018 Feb 7;6(5):781-788. doi: 10.1039/c7tb02663c. Epub 2018 Jan 19.
Herein, we focused on mucin, which is a large viscous glycoprotein in terms of materials science, and reported preparation of mucin gel particles and incorporation of enzymes to provide the particle with self-degradable and releasable properties. To expose the hydrophobic peptide cores, trimming of sugar moieties was carried out by β-elimination reaction under alkaline conditions (tMucin). Nano-sized tMucin particles were prepared by the assembly of tMucin with the aid of a cationic surfactant. Then, cross-linking of tMucin particles was carried out via heat treatment (annealing) to induce thermal aggregation of the polypeptide chains. The hydrodynamic diameter of tMucin particles reversibly changed in response to calcium ions. Next, in an attempt to render the particle degradable, lysozyme was incorporated into the tMucin particles for the hydrolysis of oligosaccharide chains. These particles were gradually degraded upon enzymatic cleavage of the mucin molecules, facilitating the release of their incorporated substances. Also, the degradation of the mucin particles and the release of lysozyme were tunable by environmental conditions, such as temperature and calcium ions, in addition to the degree of cross-linking of the particles.
在此，我们聚焦于黏蛋白，就材料科学而言，它是一种大型粘性糖蛋白，并报道了黏蛋白凝胶颗粒的制备以及酶的掺入，以使颗粒具有自降解和可释放特性。为了暴露疏水肽核心，在碱性条件下通过β-消除反应进行糖部分的修剪（tMucin）。借助阳离子表面活性剂通过tMucin的组装制备纳米级tMucin颗粒。然后，通过热处理（退火）进行tMucin颗粒的交联，以诱导多肽链的热聚集。tMucin颗粒的流体动力学直径响应钙离子而可逆地变化。接下来，为了使颗粒可降解，将溶菌酶掺入tMucin颗粒中以水解寡糖链。这些颗粒在黏蛋白分子被酶切时逐渐降解，促进其所含物质的释放。此外，除了颗粒的交联程度外，黏蛋白颗粒的降解和溶菌酶的释放还可通过温度和钙离子等环境条件进行调节。
