Vilaça Natália, Totovao Ricardo, Prasetyanto Eko Adi, Miranda-Gonçalves Vera, Morais-Santos Filipa, Fernandes Rui, Figueiredo Francisco, Bañobre-López Manuel, Fonseca António M, De Cola Luisa, Baltazar Fátima, Neves Isabel C
Centre of Chemistry, Chemistry Department, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
J Mater Chem B. 2018 Jan 21;6(3):469-476. doi: 10.1039/c7tb02534c. Epub 2018 Jan 5.
Zeolites are crystalline porous materials with a regular framework which have non-toxic effects on a variety of human cell lines and have been explored for cell imaging and drug delivery. Understanding the interaction between zeolite nanoparticles and cells is imperative for improving their potentialities, since the process of internalization of these particles is still poorly understood. In this study, the intracellular trafficking and internalization kinetics of zeolite L into breast cancer cells and normal epithelial mammary cells were analysed using scanning electron microscopy (SEM), confocal microscopy and transmission electron microscopy (TEM). We also studied the involvement of endocytic pathways using two pharmacological inhibitors, chlorpromazine and dynasore. Zeolite nanoparticles were taken up by both cell types and the cellular uptake was fast, and started immediately after 5 min of incubation. Interestingly, the uptake was dependent on the cell type since in breast cancer cells it was faster and more efficient, with a higher number of nanoparticles being internalized by cancer cells over time, compared to that in the epithelial mammary cells. TEM results showed that the internalized nanoparticles were mainly localized in the cell vacuoles. The data obtained upon using endocytic pharmacological inhibitors suggest that the zeolite L uptake is mediated by caveolin.
沸石是具有规则骨架的结晶多孔材料，对多种人类细胞系无毒作用，已被用于细胞成像和药物递送研究。了解沸石纳米颗粒与细胞之间的相互作用对于提升其应用潜力至关重要，因为这些颗粒的内化过程仍知之甚少。在本研究中，使用扫描电子显微镜（SEM）、共聚焦显微镜和透射电子显微镜（TEM）分析了L型沸石在乳腺癌细胞和正常乳腺上皮细胞中的细胞内运输及内化动力学。我们还使用两种药理抑制剂氯丙嗪和dynasore研究了内吞途径的参与情况。两种细胞类型均摄取了沸石纳米颗粒，且细胞摄取迅速，孵育5分钟后立即开始。有趣的是，摄取情况取决于细胞类型，因为在乳腺癌细胞中摄取更快且更有效，随着时间推移，与乳腺上皮细胞相比，癌细胞内化的纳米颗粒数量更多。TEM结果表明，内化的纳米颗粒主要定位于细胞液泡中。使用内吞药理抑制剂获得的数据表明，L型沸石的摄取是由小窝蛋白介导的。
