Ye Haihang, Xia Xiaohu
Department of Chemistry, University of Central Florida, Orlando, Florida 32816, USA.
J Mater Chem B. 2018 Nov 28;6(44):7102-7111. doi: 10.1039/c8tb01603h. Epub 2018 Jul 19.
Colorimetric lateral flow assay (CLFA) is one of a handful of diagnostic technologies that can be truly taken out of the laboratory for point-of-care testing without the need for any equipment and skilled personnel. Despite its simplicity and practicality, it remains a grand challenge to substantially enhance the detection sensitivity of CLFA without adding complexity. Such a limitation in sensitivity inhibits many critical applications such as early detection of significant cancers and severe infectious diseases. With the rapid development of materials science and nanotechnology, signal amplification techniques that hold great potential to break through the existing detection limit barrier of CLFA have been developed in recent years. This article specifically highlights these emerging techniques for CLFA development. The rationale behind and advantages and limitations of each technique are discussed. Perspectives on future research directions in this niche and important field are provided.
比色侧向流动分析法（CLFA）是少数几种能够真正在无需任何设备和专业人员的情况下带出实验室进行即时检测的诊断技术之一。尽管其简单实用，但在不增加复杂性的情况下大幅提高CLFA的检测灵敏度仍然是一项巨大挑战。这种灵敏度的限制阻碍了许多关键应用，如重大癌症和严重传染病的早期检测。随着材料科学和纳米技术的快速发展，近年来已开发出具有巨大潜力突破CLFA现有检测极限障碍的信号放大技术。本文特别强调了这些用于CLFA发展的新兴技术。讨论了每种技术背后的原理以及优缺点。还提供了对这一细分且重要领域未来研究方向的展望。
